Proteus Mirabilis Case Study

1217 Words 5 Pages
Proteus Mirabilis
Proteus mirabilis is defined by the slide share website as a disease caused by microbial invasion of the genitourinary track that extends from the renal cortex of the kidney to the urethral meatus. Proteus mirabilis can be laboratory diagnosed by the specimen collection, transported and storage and laboratory methods, but the laboratory methods that would be focused on is specimen collection. It is often the first determine diagnosis and treatment which would give a more detailed result. The specimen (urine) is collected. First thing specimen collection is that patients should be instructed to wash their hands. Secondly, the vulva and glans penis should be cleaned by using three swabs with soap and sterile water. Thirdly,
…show more content…
This has been brought about to improve diagnosing UTIs. Real time PCR compared well to diction by the Seegene and showed a slighter higher sensitivity of diction. The advantage of real time PCR identification of unpathogens is that results are available within a few hours. For the Conventional Diagnosis Seegene kit is used to detect these bacteria. However, it is more laborious and time-consuming since PDR produces need to be analysed by get electrophoresis. This type of diagnosis can replace culture based diagnosis. This is because there is no antibiotic sensitivity testing is required for adequate treatment of patients. Real-time PCR is by definition a least semi quantitative. Low Ct values are indicative of high loads and vice versa. Having made standard curves of the relation between loads and Ct values, quantification can also be done using higher precision. Hence instead of Ct, Cq is used. By using PCR and safely applying a cut-off value of Cq 40 at least 42% of the culturing can be eliminated. When the negative predictive value of PCA is negative and is 100% a cut off Cq value of thirty-three, the negative predictive value of PCR would be 94% mainly due to growth and Proteus Mirabilis from specimens with high or negative Cq values in PCR. The 16 PCR confirmed maximum outcomes. Only one specimen becomes discovered with gram negative PCR which became suspect to comprise any other purpose for contamination. The correlation of unique PCR and 16S became very high. Handiest while Cq values of wither Gram positive or gran negative PCR were low Cq <25, competition for response components appeared to bog down dependable detection of both, a locating which has been supplied before. This disadvantage may be triumph over by using separation of gram negative and gram positive PCR into 2 reactions. This real time PCR based diagnosis is a great way of determination of this bacteria the disease it

Related Documents