Introduction Individual microbial agents have different effects on different organisms. Some of these organisms may be completely resistant to a particular microbial while others may be highly susceptible. In this weeks experiment, the Kirby Bauer Disk Diffusion test will be used to determine if an organism is susceptible or resistant to a selection of antimicrobial agents. The outcome of this test is important for physicians in determining which antibiotics to use when treating sick patients as well as controlling the use of inappropriate antibiotics in a clinical practice. Bacteria have the ability to become resistant, therefore it is essential that advanced antibiotics are able to overcome them.
Procedure I: Kirby Bauer Creating the Bacterial Lawn …show more content…
The necessary materials needed for this experiment were collected and checked for any undesirable condition. Before inculcating the TSA plate, the surface of the agar and sides of the plate were carefully examined for any visible contamination. A bacterial culture was obtained and a sterile non-toxic swab was gently inserted inside the tube rotating around the culture. The swab was removed by carefully pressing the inside wall of the tube, removing any excess inoculum from the swab. The plate was inoculated by streaking the swab over the entire agar surface in three directions. The plate was rotated each time to ensure an even distribution of inoculum.
Procedure II: Kirby Bauer Method for Testing Antibiotic Resistance A bacterial lawn was created using one bacteria per plate. The antibiotic disk was placed on the surface of the agar using the antibiotic disc dispenser. Once the disk was placed on the agar surface, it was not to be moved. The plate was inverted into the incubator at 37 degrees Celsius.
Procedure III: Antiseptics and Disinfectants Basic Technique
A. Alcohol Flame Sterilization of
Procedure I: Kirby Bauer Creating the Bacterial Lawn …show more content…
The necessary materials needed for this experiment were collected and checked for any undesirable condition. Before inculcating the TSA plate, the surface of the agar and sides of the plate were carefully examined for any visible contamination. A bacterial culture was obtained and a sterile non-toxic swab was gently inserted inside the tube rotating around the culture. The swab was removed by carefully pressing the inside wall of the tube, removing any excess inoculum from the swab. The plate was inoculated by streaking the swab over the entire agar surface in three directions. The plate was rotated each time to ensure an even distribution of inoculum.
Procedure II: Kirby Bauer Method for Testing Antibiotic Resistance A bacterial lawn was created using one bacteria per plate. The antibiotic disk was placed on the surface of the agar using the antibiotic disc dispenser. Once the disk was placed on the agar surface, it was not to be moved. The plate was inverted into the incubator at 37 degrees Celsius.
Procedure III: Antiseptics and Disinfectants Basic Technique
A. Alcohol Flame Sterilization of