Sensitivity of Cancerous C.elegans with Mitochondria Dysfunction Mitophagy is a selective autophagic elimination of damaged or excessive mitochondria, especially when cells are under adverse stress. Mitophagy has been identified as the cause of various human diseases and even cancer. In recent years, researchers gradually regard mitophagy as the target for cancer treatment, although the role of mitophagy in the developmental and metabolic process of cancer is still not clear. In this experiment, we used C. elegans as a model for mitochondria dysfunction drug treatment to test whether drugs have different effects on wild type and cancerous C.elegans.
Introduction
Mitochondria dysfunction and mitophagy In yeast and mammalian cell, mitochondria serve as a dynamic machine to provide ATP for cell metabolism through oxidative phosphorylation pathway. When cells under adverse environment, damaged or dysfunctional mitochondria tend to stop producing ATP but release ROS, which will be accumulated to kill cells1. …show more content…
During the process of mitophagy, damaged and excessive mitochondria would be confined within autophagosome and digested by lysosomal hydrolases 2. PINK-1, which was knocked down by RNAi of mutant in following experiment, is kinase for Parkin-dependent mitophagy pathway. PINK-1 with its downstream protein PARKIN, can find and specifically bind to the dysfunctional mitochondria. The kinase activity of PINK-1 can depolarize damaged ones and recruit other hydrolyte for
Introduction
Mitochondria dysfunction and mitophagy In yeast and mammalian cell, mitochondria serve as a dynamic machine to provide ATP for cell metabolism through oxidative phosphorylation pathway. When cells under adverse environment, damaged or dysfunctional mitochondria tend to stop producing ATP but release ROS, which will be accumulated to kill cells1. …show more content…
During the process of mitophagy, damaged and excessive mitochondria would be confined within autophagosome and digested by lysosomal hydrolases 2. PINK-1, which was knocked down by RNAi of mutant in following experiment, is kinase for Parkin-dependent mitophagy pathway. PINK-1 with its downstream protein PARKIN, can find and specifically bind to the dysfunctional mitochondria. The kinase activity of PINK-1 can depolarize damaged ones and recruit other hydrolyte for