The addition of the succinate was required in order for the DPIP to be reduced. The addition of the succinate did impact the reactions, however too much the succinate will not be as successful because based on Table 1.4, it was shown that the Sample ID “2” had 100μL of the succinate and it had the reaction rate of 0.68 %/min. However, with the excess amount of succinate which was 200 μL with a reaction rate of 0.0533%/min. However, no addition of succinate could definitely lessen the reaction rate as Sample ID “1” had 0.0267%/min. The mitochondria are respiring because the Sample IDs “1”, “2”, “3” and “4”. Sample IDs “1”, “2”, “3” all contained mitochondrial suspension. However, Sample ID “4” did not contain any mitochondrial suspension and
