A single Lactaid pill was grinded with a mortar and pestle.
120 mg of the ground up Lactaid pill was dissolved in 200 mL of 0.08 M phosphate buffer (7.7 pH) and the resulting solution was centrifuged to remove any capsule remains.
Enzyme and Substrate Solution Preparation
A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm.
The substrate solutions were prepared individually by adding 4.5 mL 0.08 M phosphate buffer (pH 7.7) to a Spec 20 cuvette with 0.5 mL of ONPG. The enzyme solution was prepared according to enzyme isolation process.
Enzyme Assay
0.5 mL of the isolated enzyme was quickly added to the prepared,
120 mg of the ground up Lactaid pill was dissolved in 200 mL of 0.08 M phosphate buffer (7.7 pH) and the resulting solution was centrifuged to remove any capsule remains.
Enzyme and Substrate Solution Preparation
A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm.
The substrate solutions were prepared individually by adding 4.5 mL 0.08 M phosphate buffer (pH 7.7) to a Spec 20 cuvette with 0.5 mL of ONPG. The enzyme solution was prepared according to enzyme isolation process.
Enzyme Assay
0.5 mL of the isolated enzyme was quickly added to the prepared,