Lab 1: Microscopy and the Metric System Essay

862 Words Feb 12th, 2013 4 Pages
Lab 1: Microscopy and the Metric System
Purpose
The purpose of this lab is to become familiar with the lighted microscope, how to prepare a wet mount, and understand taking measurements in the metric system and its conversions.

Materials & Methods Materials:
Part A: * Computer for videos

Part B: * Tape measure with centimeters * Scale that measures grams * Thermometer that shows Celsius * Campbell Biology Book * 1 piece Orange Starburst

Methods: Part A: Combined Lighted Microscope 1. Watch video on microscope 2. Answer the provided questions on the microscope
Wet Mount: 1. Watch video on wet mount 2. Explain the process of creating a wet mount Part B: Width of Book: 1. Lay book
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**With a 10x viewing lens h) 4x- 40x i) 10x- 100x j) 40x- 400x k) 100x- 1000x 2) What is meant by the depth of field? l) How sharp you can view the object on the slide 3) What is meant by the field of view? m) The amount you can see on the slide without moving it. 4) Describe the process of making a wet mount. n) Wet Sample- First you take the dropper to pick up the sample material and apply a small amount on the glass slide. Then take a cover glass and carefully lower it on top of the sample. Also if there is excess water, use filter paper to absorb it. And if not enough water then place a small amount on the edge of the cover glass and it will suck it under the cover glass. o) If the sample is too big- Place two drops of water on each end of the slide and place a cover glass on top of each drop. Then place a cover glass on top of the ends of the two cover glasses. Then place the sample under the third cover glass with a pipette. Also if there is excess water, use filter paper to absorb it. And if not enough water then place a small amount on the edge of the cover glass and it will suck it under the cover glass. p) Dry Sample- First to place some water (or immersion oil) on the slide and then put the dry sample on top of it. Then you place more water on top of the dry sample. Also if there is excess, water use filter paper to absorb it. And if not enough water then place

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