| IDENTIFICATION OF SOME MACROMOLECULES |
INTRODUCTION Dehydration-synthesis is a reaction that occurs to form different types of macromolecules. It is a reaction that leaves a macromolecule structure along the lines of developing its own specific function in a living organism. The 4 most common ones are carbohydrates which includes monosaccharides and polysaccharides, lipids, proteins and nucleic acids (Mack 2012). Scientists identify different types of macromolecules to achieve a better understanding on how they function in our bodies. This can be achieved by studying their chemical properties and structure.
A few experiments were
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One major difference between starch and glycogen is the branching in the final polysaccharide structure. Starch comes from the mixture of amylose and amylopectin. Since amylose is unbranched with a helical molecule shape, only a 1-4 alpha linkage is possible. But because amylopectin is branched with a straight chain, it can have both 1-4 and 1-6 alpha linkages. (http://www.scribd.com/doc/20872349/Starch-and-Glycogen) The Benedict’s test was used to test for reducing sugars. Reducing sugars act as reducing agents during the Benedict’s test which means they give away electrons during a redox reaction. This came from the carbohydrate macromolecule since sugars are the basic of carbohydrate molecules. Two forms of sugar can exist: a straight chain and a ring form. In order to determine if a reducing sugar is present, the solution must be boiled with some Benedict’s solution. If colours ranging from yellow or green, to red or brown occur, the test is positive. Otherwise the test is negative. CuSO4, sodium carbonate and sodium citrate are all in the Benedict’s solution.
The biuret test was used to test for proteins. It uses CuSO4 blue solution to help determine the presence of proteins. If the solution turns from blue to violet, the result is positive. If there is no change and blue solution