How Optimized Pf0 Encapsulated Hybrid Ncs Formulation Was Achieved By A Two Step Process
2193 Words Nov 17th, 2016 9 Pages
In Step II, PF0-HNC was prepared by coating chitosan on the optimized PF0-ANC by solvent gelation technique. The PFC-ANC was allowed to interact with chitosan for a period of 2 hours. The chitosan coated BSA NCs were subsequently stabilized by TPP. The concentrations of chitosan and TPP were selected as described in our previous publication . The optimized concentration of chitosan (0.125 %w/v) and TPP (0.125 %w/v)] were used to prepare PF0-HNC’s. Finally, these HNC’s stabilized with TPP were collected by centrifugation (16,000 g; 30 min) and lyophilized using 2 %w/v lactose as cryoprotectant for future use.
Figure 1: Illustration of the targeted PF0 loaded hybrid nanocarrier (PF0-HNC-E) preparation process. The first step involves formation…