How Optimized Pf0 Encapsulated Hybrid Ncs Formulation Was Achieved By A Two Step Process

2193 Words Nov 17th, 2016 9 Pages
The optimized PF0 encapsulated hybrid NCs formulation was achieved by a two-step process (Fig 1) using nanoprecipitation and subsequently coating with chitosan. In Step-I, the nanoprecipitation technique was used to prepare PF0 loaded BSA NCs (PF0-ANC). In this nanoprecipitation technique, acetone was used as a desolvent [40]. Varying concentrations of PF0 solutions (125µM - 375µM) and BSA solution (156.25µM – 468.75µM) (Table 1) were mixed in a glass vial. The PF0 BSA solution was then added slowly, at a controlled speed of 0.2 ml/min using syringe pump, under continuous stirring at 600 rpm and the stirring was continued overnight to allow acetone evaporation. The NCs obtained in the above step was used for the future steps.
In Step II, PF0-HNC was prepared by coating chitosan on the optimized PF0-ANC by solvent gelation technique. The PFC-ANC was allowed to interact with chitosan for a period of 2 hours. The chitosan coated BSA NCs were subsequently stabilized by TPP. The concentrations of chitosan and TPP were selected as described in our previous publication [24]. The optimized concentration of chitosan (0.125 %w/v) and TPP (0.125 %w/v)] were used to prepare PF0-HNC’s. Finally, these HNC’s stabilized with TPP were collected by centrifugation (16,000 g; 30 min) and lyophilized using 2 %w/v lactose as cryoprotectant for future use[41].

Figure 1: Illustration of the targeted PF0 loaded hybrid nanocarrier (PF0-HNC-E) preparation process. The first step involves formation…

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