A DNA ladder is a solution of DNA molecules of different but known length. In Well 1, the DNA ladder was applied to the gel to set a standard for the rest of the samples. In LoggerPro, after setting an origin, the second step was to set a standard ladder. We standardized the ladder by going from 4 to 5 cm and determining that was 10 milliliters. The next step was to scale the ladder by selecting the 3 brightest points and labeling it 3000,1000,500 in descending order.
The estimated size of my genomic from the PCR amplicon amplified from the D1S80 locus is 465 base pairs. On LoggerPro, my group took the 3 brightest points on the standard ladder to estimate 3000 base pairs, 1000 base pairs, and 500 base pairs. Using that, my group pin pointed the …show more content…
Rounding up, the number of repeats that were for my D1S80 locus was 20 repeats. My genotype is homozygous, this was determined in the picture in question 5. In Lane 4, there was only one band of PCR amplicon which caused me to guess that my genotype was homozygous.
Restriction enzymes are enzymes that occur naturally within the bacteria that cut DNA molecules into small fragments. They are called “restricted” enzymes because the enzyme only recognizes a specific DNA sequence. This is the area where it binds and then cuts the double-stranded DNA. An example of how this can be used by research scientists is identifying individual or several strains of a desired species. Gel electrophoresis separates DNA fragments which can be used to distinguish types of bacteria. This can be used to identify whether or not a specific type of bacteria caused a disease outbreak.
The difference between a single digest and a double digest is that for a double digest, you should see 2 fragments while the single digest you should only see 1 fragment. They both produce linear fragments and both linear form runs according to size in
The estimated size of my genomic from the PCR amplicon amplified from the D1S80 locus is 465 base pairs. On LoggerPro, my group took the 3 brightest points on the standard ladder to estimate 3000 base pairs, 1000 base pairs, and 500 base pairs. Using that, my group pin pointed the …show more content…
Rounding up, the number of repeats that were for my D1S80 locus was 20 repeats. My genotype is homozygous, this was determined in the picture in question 5. In Lane 4, there was only one band of PCR amplicon which caused me to guess that my genotype was homozygous.
Restriction enzymes are enzymes that occur naturally within the bacteria that cut DNA molecules into small fragments. They are called “restricted” enzymes because the enzyme only recognizes a specific DNA sequence. This is the area where it binds and then cuts the double-stranded DNA. An example of how this can be used by research scientists is identifying individual or several strains of a desired species. Gel electrophoresis separates DNA fragments which can be used to distinguish types of bacteria. This can be used to identify whether or not a specific type of bacteria caused a disease outbreak.
The difference between a single digest and a double digest is that for a double digest, you should see 2 fragments while the single digest you should only see 1 fragment. They both produce linear fragments and both linear form runs according to size in