Unit 4 Fossil Blast Lab

Improved Essays
Five morphological observations were made from looking at the unknown fossil specimen. See the list of the observations that were made. Figure 4 Fossil Cladogram was then redrawn into the lab journal and a prediction was made on the fossil placement on the cladogram based on the observations. Next, an explanation of the reasons for the prediction was then recorded in the lab notebook. The four gene file were then downloaded onto the computer by going to the website listed below. Since the gene files will not open on the computer, a folder that was titled, “BLAST Lab” was created to put the files in. Once the files have been downloaded and stored in a folder, the next step was to go to the BLAST home page. Selecting the link, “Saved Strategies” …show more content…
No changes or alterations were not allowed to be made once the page that contains the parameter appeared. When that page appeared, BLAST button (located at the bottom of the page) was then selected. Two sections appeared on the page. The first section is a graphic display of the matching sequence, which will not be needed in this portion of the lab. The second part of the page, “Sequences producing significant alignments” showed a list of genes. The first link on the list for was used for all of the four genes to find the identity of the gene because it was the most similar. When clicking on a particular species that was listed, a full report of the specie appeared on the page. The information on the report was then used to fill out the BLAST Data card for each gene files. The genus and specie was the first two words in the name, and the common name was found by googling the genus and specie name. The protein produce by the sequence was found by looking at the third word of the link. By clicking on the Distance Tree Results link at the top of the page, it showed a cladogram for that particular species. The option, “Slanted” was chosen to aid in the process of drawing a cladogram for the distance tree

Related Documents

  • Improved Essays

    PCR Amplification Desired DNA was amplified in 200µL PCR tubes. WtfolA PCR tubes contained 0.1584ng/µL wildtype folA derived from pMAC1-wtfolA (biochemistry teaching labs), 0.2µM forward primer (MOBIX, CGGCAGCCATATGATCAGTCTGATTGCGGC) and 0.2µM reverse primer (MOBIX, GTGCTCGAGCCGCCGCTCCAGAATCT). MutfolA PCR tubes contained 4ng/µL mutant folA derived from pET28b-mutfolA (biochemistry teaching labs), 0.2µM forward primer (MOBIX, GACGGACACATATGATCAGTCTGATTGCGGCG) and 0.2µM reverse primer (MOBIX, ATATACTCGAGCCGCCGCTCCAG). Each tube contained 1X PCR buffer (iNtRON Biotechnology, FroggaBio; 100mM Tris-HCl pH8.3, 500mM KCl, 20mM MgCl2), 10mM dNTP mixture (iNtRON Biotechnology, FroggaBio, 2.5mM each of dATP, dCTP, dGTP, dTTP), 0.05U/µL i-Taq™ DNA polymerase (iNtRON Biotechnology, FroggaBio) and nuclease free water. PCR tubes were placed in an Eppendorf Mastercycler and programmed as follows: 95°C for 5…

    • 742 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    Fossil Es3227 Analysis

    • 128 Words
    • 1 Pages

    Fossil ES3227 The Fossil Georgia Collection was a big hit around the world; the timepieces made under this collection are chic, trendy, and sophisticated. Adding to Fossil's popular collection is Fossil ES3227. This watch is perfect for every woman who loves sophistication and simplicity at the same time. Fossil ES3227 has round gold-tone 32 mn stainless steel case protected with thick mineral crystal, adding sophistication to its look is a white mineral dial embellished with crystal…

    • 128 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    For the sequencing reaction protocol two new Eppendorf tubes were used one was labeled ND4 and the other Leu. Using a new pipette, two microliters of stock PCR product were added to each tube. Then 5.5µL of ddH2O was added to each tube for a total 7.5µL of mix in each tube. Next, 0.5µL ND4 primer was added to the tube labeled ND4 and 0.5µL Leu primer was added to the tube labeled Leu. Two microliters of Big Dye Terminator Cycle…

    • 776 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    Geology Camp Archeology

    • 166 Words
    • 1 Pages

    Attending the geology camp GeoFORCE for the past four years, I have learned a quite a few things about erosion, archeology, and the affects of the erosion in the future. Erosion is a scary subject to talk about and the fact that the North Slope’s erosion is one of the fastest rates of erosion in the nation scares me to death. However, the erosion revealed some fascinating human remains. With my little knowledge of archeology and geology, the law of uniformitarianism, which means the present is the key to the past, comes to play. The remains that were found can unveil some things we did not know about the past.…

    • 166 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Eukaryotic Cells

    • 741 Words
    • 3 Pages

    The aim of the data analysis exercise was to determine genetic relationships between the HSP70 amino acid chains of fourteen different organisms. The data produced was an identification table, a sequence alignment diagram and a cladogram. In cladograms the branches are not representative of the evolutionary time that has occurred. Heat shock proteins (HSPs) are in a class of proteins called molecular chaperones. (Kiang, J.; G, Tsokos. 1998).…

    • 741 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Bsmutl Model Summary

    • 721 Words
    • 3 Pages

    The overall purpose of the study was to create a model based off the crystal structure of the endonuclease domain of Bacillus subtilis MutL. This structure, in particular, exposes the presence of zinc in the binding site which is important for the MMR function of BsMutL in vivo. From there, they proposed a model describing the association of MutS and DNA polymerase III, β clamp with MutL, and nicking of a synthesized DNA strand. The importance of MutL is in its united effort with MutS to recognize mismatches and interactions of the β clamp - these correlate with the polymerases ability to catalyze consecutive reactions without releasing the substrate. The combined effort could allow MutL to signal nicking of a newly synthesized DNA strand.…

    • 721 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Why Are Fossils Important

    • 389 Words
    • 2 Pages

    Fossils are considered essential for the study of biological evolution because they are important in creating a time frame for once living organisms. They are the only specimen that provide us with direct physical evidence of the existence of past life as well as showing us the proof of evolution through the changes of each fossil’s skeletal structure. Sometimes interpreting the sequence of evolution within a living creature is difficult as the full range of fossils may not have been found, meaning that we may not have been able to see the entire evolutionary process. Phylogeny, which is the biological change and evolutionary relationships is documented through the place of fossils in time. There are two main ways out finding out how old something:…

    • 389 Words
    • 2 Pages
    Improved Essays
  • Superior Essays

    Analysis Of HCV DNA

    • 975 Words
    • 4 Pages

    For each target RNA, 2 PNA probe pairs [G-probe with C-terminus FITC and A-probe (antigen, or biotin or antibody)] will be designed and evaluated. Probe design will be performed via Primer Express version two software. The software will create probe sets designed. Probe hybridization sequences will be evaluated by BLAST to select regions conserved across multiple transcript variants. For initial design, target complexity and accessibility will be evaluated using Visual OMP (DNA Software Inc., Ann Arbor, MI).…

    • 975 Words
    • 4 Pages
    Superior Essays
  • Improved Essays

    AK1 Genome Study

    • 961 Words
    • 4 Pages

    flavithermus AK1 was obtained from the NCBI server (http://www.ncbi.nlm.nih.gov/assembly/GCF_000353425.1/and http://www.ncbi.nlm.nih.gov/Traces/wgs/?val=APCD01). Afterwards, the genomic circular map was generated by usingClicO Free Service (ClicO FS), an online web-service based on Circos[17]. Briefly, 3 text files were prepared in order to run in the online server, i.e., a seven columnskaryotype file containing information of 50 contigs; a three columns forward genes file containing information on contigs ID, coordinate start, and coordinate end; and a three columns reverse genes file containing information on contigs ID, coordinate start, and coordinate end. The files were uploaded to the server and on-screen instructions were followed which generated Circos circular plot for the…

    • 961 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    Bwok Analysis

    • 982 Words
    • 4 Pages

    The study is aimed at the comparison of homologous protein BLOCKs using different diversity parameters (MDRs, DHPs and MCRs etc) that are formulated using positional frequencies of observed hetero-pairs and homo-pairs of BLOCKs. APBEST, written in AWK programming language, extracts these BLOCK specific parameters. How efficient is the program? Are these parameters correlate with already existing literature reports? To have resolution of these questions, we have implemented the program first on constructed BLOCK FASTA files.…

    • 982 Words
    • 4 Pages
    Great Essays
  • Improved Essays

    It has a vast and rich database that contains current and archived genome assemblies of many organisms. The browser tool allows zoom in and look in detail of each chromosomes with detail annotation. The gene sortor tool allows to look for gene expression and homology. The BLAT tool allows in sequence alignment directly to desired genome [4]. “The UCSC genome browser provides a visualization interface that renders the genome sequence data as linear sequence at varying levels of granularity and allows the viewer to navigate along the length of the sequence, jump to specific locations in the genome using genomic coordinates, or zoom in and out do varying levels of detail” [2].…

    • 1066 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Scientists classify organisms by grouping and sorting organisms together based on their physical structure, evolutionary relationships, embryonic similarities, genetic similarities, and their biochemical similarities. The most popular form of classification system used by scientists is Linnaeus’s System of Classification, by which organisms are classified and grouped into 6 different kingdoms; bacteria, archaea, protista, fungi, plant and animal. By classifying organisms it provides scientists with an easy way to study organisms efficiently, and allows for predictions and knowledged observations. Knowledge about classification allows scientists to make predictions about organisms, living and extinct. It allows for a comparison and understanding…

    • 677 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Multiplex PCR

    • 1066 Words
    • 5 Pages

    After each cycle, the newly synthesized DNA strands can serve as template in the next cycle. 4.2. Advantages of Multiplex Polymerase Chain Reaction Since its first description in 1988, multiplex PCR has been successfully applied in many areas of DNA testing including analyses of deletion, mutations, and polymorphisms, or quantitative assays and reverse transcription (Henegariu et al., 1997). Indeed, this technique has several advantages such as increasing the number of genes that can be analyzed, reducing sample requirement, saving time, effort, and saving potentially money. Furthermore, using multiplex PCR, the same reaction conditions can be provided for several targets.…

    • 1066 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Polymerase Chain Reaction

    • 1271 Words
    • 6 Pages

    Polymerase chain reaction (PCR) Generalities PCR is a method developed in 1985 by Kary Mullis and to obtain, by coupling a heat-resistant DNA polymerase and without cloning, amplification of a fragment of DNA known. Initially, DNA polymerase was isolated from a bacterium thermophilne (resistant to significant increases in temperature), as Thermus aquaticus (Taq polymerase). Currently, we use recombinant enzymes, whose; elaboration is easier and greater efficiency. The general principle of PCR is the in vitro amplification of a specific area of a nucleic acid.…

    • 1271 Words
    • 6 Pages
    Improved Essays
  • Improved Essays

    Triple Taqman Essay

    • 761 Words
    • 4 Pages

    In each batch, the known genotype of vanA, vanB, vanM strains are used as positive control, ATCC29212 and saline are used as negative control and blank control, respectively. 20 μL vanA, vanB, vanM primer reaction system consists of 1 μL upstream primer, 1 μL downstream primer, 10 μL 2 ofTaq PCR MasterMix (KT201), 6 μL sterile ultrapure water and 2 μL DNA template. VanA, vanB, vanM primer N-VAN and other reaction sequences and reaction procedures are listed in Table 2.All PCR products were sent to sequencing. Each batch of reactions contained a negative control, a positive control, a blank control, which were identical to the triple Taqman probe real-time PCR…

    • 761 Words
    • 4 Pages
    Improved Essays