Introduction:
Bacteria and Fungi are important contributors of the living life. They however differ in their colony/ color/ size/shape/appearance. Bacteria are generally small in size but large in colonial structure, they are usually in the form of coccus, bacillus and spirillum, many times bacteria are of vivid colors because they have chlorophyll which also absorbs the light of different wavelengths which create natural colors of brown, pink, blue, orange and purple. Fungi on the other hand do not have chlorophyll, they are bigger in size, and they can be found usually in the form of yeast, mold and mushrooms. Which have most of the colors. When the environment is nutritious the bacteria and fungus can grow again. Both are very resistant …show more content…
- Dirt
- Agar Plate
- Ciproxin
- Yogurt
The General Method (Second Class Time)
1. We spread 3 kinds of bacteria on the slide using a toothpick and a drop of water.
2. For each specie we used different toothpicks and put three water drops.
3. By using an alcohol flame lamp we fixed the bacteria to the slide.
4. Added a drop of the fuschin to each of the three species and warmed the slide over the alcohol flame.
5. We did destain (D) the slide by using a few drops of acid-alcohol.
6. Used the gentle steam of water to rinse off.
7. We added a drop of Methylene Blue Solution and did let it to sit for a minute.
8. Rinsed off the Methylene Blue Solution with the tap water.
9. Then after rinsing the Methylene Blue Solution off we dried the slide in the air.
10. We started to view the bacteria in the microscope from the lowest power lens and then increased it till the power rotating the lens of the objective.
11. Added a drop of immersion of oil to each of the three species.
12. Detected the bacteria which was with the immersion oil lens which we have seen in the microscope.
Group A: Environmental …show more content…
Method:
1. Dry swabs of eye corners
2. Moist swab of the behind of an ear
3. Moist swab of the foot between toes
4. Moist swab of skin on the back of the hand
The Result: In the following picture we can observe an astonishing phenomenon we can observe Fig. 1.8 In the above picture we can see how rapidelly this organism has divided itself and made more of itself. In this picwe can observe how rapidely fungi grew.
Group E: Enviornmental sample-Dirt Sample
Hypothesis: There is a bigger chance that we will find fungi.
1. We went outside and collected a small scoop of dirt and put in a test tube
2. Then we added ten ml of sterile water onto the tube mixed substances
3. We removed 1 ml of this mixture and added 9 ml of water to it
4. We did the above process 3 times until we got 3 dillutions (1, 1/10 and 1/100)
5. Then we placed 1 ml of these delutions into the agar plate, as instructed we waited and let the water sink in the agar
6. Finally we moved a piece of paper with Ciproxin (which is an antibiotic for the treatment of bacterial infections) antibiotic in the middle of the first dilution
Bacteria and Fungi are important contributors of the living life. They however differ in their colony/ color/ size/shape/appearance. Bacteria are generally small in size but large in colonial structure, they are usually in the form of coccus, bacillus and spirillum, many times bacteria are of vivid colors because they have chlorophyll which also absorbs the light of different wavelengths which create natural colors of brown, pink, blue, orange and purple. Fungi on the other hand do not have chlorophyll, they are bigger in size, and they can be found usually in the form of yeast, mold and mushrooms. Which have most of the colors. When the environment is nutritious the bacteria and fungus can grow again. Both are very resistant …show more content…
- Dirt
- Agar Plate
- Ciproxin
- Yogurt
The General Method (Second Class Time)
1. We spread 3 kinds of bacteria on the slide using a toothpick and a drop of water.
2. For each specie we used different toothpicks and put three water drops.
3. By using an alcohol flame lamp we fixed the bacteria to the slide.
4. Added a drop of the fuschin to each of the three species and warmed the slide over the alcohol flame.
5. We did destain (D) the slide by using a few drops of acid-alcohol.
6. Used the gentle steam of water to rinse off.
7. We added a drop of Methylene Blue Solution and did let it to sit for a minute.
8. Rinsed off the Methylene Blue Solution with the tap water.
9. Then after rinsing the Methylene Blue Solution off we dried the slide in the air.
10. We started to view the bacteria in the microscope from the lowest power lens and then increased it till the power rotating the lens of the objective.
11. Added a drop of immersion of oil to each of the three species.
12. Detected the bacteria which was with the immersion oil lens which we have seen in the microscope.
Group A: Environmental …show more content…
Method:
1. Dry swabs of eye corners
2. Moist swab of the behind of an ear
3. Moist swab of the foot between toes
4. Moist swab of skin on the back of the hand
The Result: In the following picture we can observe an astonishing phenomenon we can observe Fig. 1.8 In the above picture we can see how rapidelly this organism has divided itself and made more of itself. In this picwe can observe how rapidely fungi grew.
Group E: Enviornmental sample-Dirt Sample
Hypothesis: There is a bigger chance that we will find fungi.
1. We went outside and collected a small scoop of dirt and put in a test tube
2. Then we added ten ml of sterile water onto the tube mixed substances
3. We removed 1 ml of this mixture and added 9 ml of water to it
4. We did the above process 3 times until we got 3 dillutions (1, 1/10 and 1/100)
5. Then we placed 1 ml of these delutions into the agar plate, as instructed we waited and let the water sink in the agar
6. Finally we moved a piece of paper with Ciproxin (which is an antibiotic for the treatment of bacterial infections) antibiotic in the middle of the first dilution