Plasmids And E. Coli Growth

After analyzing the results obtained, one can state that the data provided gives us enough evidence to support that the plasmids without any ampicillin had the highest levels of E.coli growth, while the plasmids with ampicillin had the lowest levels of E.coli growth. This represents that there is enough evidence to reject the null hypothesis, stating how there is no correlation between the plasmids and the ampicillin present. There’s enough evidence to support the alternative hypothesis, which states that there is a correlation between the plasmids and the ampicillin present in the bacteria. The results indicated in Figure 9.1, represent how in the six agar plates obtained, three of them had ampicillin, while the other three had no ampicillin. …show more content…
In the agar plates containing both pUC18 and lux various colonies were observed. This showed the effect of the plasmid on E.coli survival in an antibiotic environment. Each colony was formed by the survival and reproduction of a single cell (Singer 2014). As the cells in both agar plates continued to replicate, so did the plasmids, providing every cell with the gene necessary to survive in the same environment (Alberte et al., 2012). The plates containing no plasmids showed no colonial growth; all E.coli cells were killed by the antibiotic before they were able to reproduce. This made it possible to tag the cells, making it clear that only those cells that replicated had been transformed (Singer 2014). The three plates containing no ampicillin, showed stable patchy growth leaning towards lawn growth. If the plates had been left at their optimal temperature for about a day longer, lawn growth was highly likely to be