It is also important to get information about changes in population, and characteristics of the cases involved. Our method of determining if the cases were related was by performing an DNA fingerprinting by Pulsed field gel electrophoresis. In DNA fingerprinting, bacteria is cut and the pieces are separated by placing in jelly-like substance. Smaller pieces move through the gel quicker than larger pieces which results in separation of DNA into distinct bands.If the DNA is different it will result in a different banding patterns but based on figure one in appendix E five out of the seven isolates were indistinguishable from each other. The DNA fingerprinting we performed showed that 17 of the first 19 E.coli isolates were identical and did not match any isolates from E.coli cases before
It is also important to get information about changes in population, and characteristics of the cases involved. Our method of determining if the cases were related was by performing an DNA fingerprinting by Pulsed field gel electrophoresis. In DNA fingerprinting, bacteria is cut and the pieces are separated by placing in jelly-like substance. Smaller pieces move through the gel quicker than larger pieces which results in separation of DNA into distinct bands.If the DNA is different it will result in a different banding patterns but based on figure one in appendix E five out of the seven isolates were indistinguishable from each other. The DNA fingerprinting we performed showed that 17 of the first 19 E.coli isolates were identical and did not match any isolates from E.coli cases before