Chromatography, Chromatography And Chromatography Essay

1030 Words Nov 14th, 2015 null Page
Introduction Separation, a process by which the distinguishing between substances through physical or chemical means in a mixture may be carried out. A widely employed technique for this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be using specific types of chromatography, termed Thin-Layer Chromatography (TLC) and column chromatography. Essentially, TLC is an adsorption aspect of chromatography that uses a polar stationary phase and a non-polar mobile phase, and utilizes capillary action to cause the solute to move or interact with the polar stationary phase. 1 It uses the attraction of like molecules (non-polar to non-polar and polar to polar attraction) as a means of separation of mixtures in order to identify what they are by use of their retention factor (Rf). 1 The structure, which influences the polarity of a substance, therefore, has a crucial role in identifying the mixtures identity. This technique along with column chromatography will be used to separate the constitutional isomers of nitroaniline (Figure 1 and Figure 2). The use of column chromatography is due to its purifying qualities. It has the ability to separate components from a mixture. This relates back to the aim of the experiment, as…

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