Methods
1. Treatment of barley with endophyte. • Five types of Barley were treated with the endophyte Pseudomonas fluorescens L321.
• They were innoculated with the seed coating mixture ( 4% sodium alginate and 1% skimmed milk powder) without the bacteria. The bacteria were grown up overnight in LB broth supplemented with 3% trehalose (400ml per strain) , centrifuged and then added and then resuspended in the coating mixture.
• 30ml of inoculant mixture was then added to 1kg of seed in a plastic sandwich bag and mixed to ensure all seeds were coated and then allowed to air dry for 3 hours.
2. Preparation of site.
• A suitable site was decided on where we would grow the barley. Darraghs farm was most suitable as it …show more content…
Measurement and results.
• The first form of data collection was the amount of seeds germinated per plot. We used a quadrat for this. A quadrat was randonly thrown on the plot of barley and the amount of germinated seeds inside the quadrat were counted. The result was multiplied by 10 because the quatrat was 10 times smaller than the size of the actual plot. This method was used for the remaining 79 plots. All of the collected data was inputted in to an excel spreadsheet. The ‘Amount of germinated seeds per plot’ was counted every week.
• The second item of data that was collected was percentage ground cover. An application called ‘Canopeo’ was used to calculate percentage ground cover. An iPad was held over the plot, and a picture was taken on the canopeo app. The app then calculated the percantage ground cover. This was done for each of the eighty plots. ‘Percentage ground cover’ was measured once every week. The data collected was inputted into an excel spreadsheet.
• Two weeks prior to harvesting the amount of heads per plot were counted. A quadrat was used when this was being counted. We threw the quatrat on a random place on the each plot. The result was multiplied by 10 as the quadrat was 10 times smaller than the actual size of the …show more content…
This was decided because the barley would heat when it would be bagged if it was damp. Firstly the netting covering the barley had to be removed. Paper bags were purchased from the local miller. Paper bags were got so that the paper would absorb any moisture from the barley. We were advised to use a petrol hedger to cut the barley. One person held on to the barley when was was being cut and another held the labelled bag open for third person to put the barley into it. It took approx. 4/5 hours to harvest the barley. When the barley was bagged it was put it into a dry shed. Take. It was necessary to take the barley out of the trailer when it was in the shed as it would have still heated when it was in the
1. Treatment of barley with endophyte. • Five types of Barley were treated with the endophyte Pseudomonas fluorescens L321.
• They were innoculated with the seed coating mixture ( 4% sodium alginate and 1% skimmed milk powder) without the bacteria. The bacteria were grown up overnight in LB broth supplemented with 3% trehalose (400ml per strain) , centrifuged and then added and then resuspended in the coating mixture.
• 30ml of inoculant mixture was then added to 1kg of seed in a plastic sandwich bag and mixed to ensure all seeds were coated and then allowed to air dry for 3 hours.
2. Preparation of site.
• A suitable site was decided on where we would grow the barley. Darraghs farm was most suitable as it …show more content…
Measurement and results.
• The first form of data collection was the amount of seeds germinated per plot. We used a quadrat for this. A quadrat was randonly thrown on the plot of barley and the amount of germinated seeds inside the quadrat were counted. The result was multiplied by 10 because the quatrat was 10 times smaller than the size of the actual plot. This method was used for the remaining 79 plots. All of the collected data was inputted in to an excel spreadsheet. The ‘Amount of germinated seeds per plot’ was counted every week.
• The second item of data that was collected was percentage ground cover. An application called ‘Canopeo’ was used to calculate percentage ground cover. An iPad was held over the plot, and a picture was taken on the canopeo app. The app then calculated the percantage ground cover. This was done for each of the eighty plots. ‘Percentage ground cover’ was measured once every week. The data collected was inputted into an excel spreadsheet.
• Two weeks prior to harvesting the amount of heads per plot were counted. A quadrat was used when this was being counted. We threw the quatrat on a random place on the each plot. The result was multiplied by 10 as the quadrat was 10 times smaller than the actual size of the …show more content…
This was decided because the barley would heat when it would be bagged if it was damp. Firstly the netting covering the barley had to be removed. Paper bags were purchased from the local miller. Paper bags were got so that the paper would absorb any moisture from the barley. We were advised to use a petrol hedger to cut the barley. One person held on to the barley when was was being cut and another held the labelled bag open for third person to put the barley into it. It took approx. 4/5 hours to harvest the barley. When the barley was bagged it was put it into a dry shed. Take. It was necessary to take the barley out of the trailer when it was in the shed as it would have still heated when it was in the