Bacterial Staining Protocols For The Visualization Of The Cells Morphology And Identifying Characteristics

836 Words Sep 29th, 2016 4 Pages
Bacterial staining protocols allow for the visualization of the cells morphology and identifying characteristics. In this experiment, Klebsiella Pneumoniae was stained in order to determine the strains shape, size, arrangement and motility. A methylene blue simple stain and negative stain allowed for the shape, size and arrangement of the cells to be observed. The hanging drop test observed how the bacterium moved. The results from the stains revealed that the Klebsiella Pneumoniae was rod-shaped and encapsulated. The individual cells also were not clustered together. The purpose of this experiment was to use staining techniques to characterize a given bacterium sample. All of the stains were successful, and were accurate compared to the actual characteristic of the bacterium.
Introduction:
Bacterium staining protocols have become universally common in research and experiments. Staining techniques allow for microorganism size, shape, motility and proximate arrangement to be assessed. These characteristics are important in identifying diseases and differentiating cells to create a comparative view of different microorganisms. There are several staining procedures that exist, however this lab focuses on the hanging drop test, simple stain and negative stain protocol. The hanging drop test allows for the observation of motility while the organism is living. A simple stain, more specifically a methylene blue stain is used for determining morphology. The presence of negatively…

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