Essay about Ap Biology

3284 Words Dec 4th, 2011 14 Pages
* DNA technology – Methods for studying and manipulating genetic material, has rapidly revolutionized the field of forensics, the scientific analysis of evidence for legal investigators * Has practical applications beyond its use in forensic science * Include the use of gene cloning in the production of medical and industrial products, the development of genetically modified organisms for agriculture, and even the investigation of genealogical questions * DNA evidence was used to solve a double murder in England * Showed that two murders could have been committed by the same person * Showed the innocence of someone who confessed to one of the murders * Showed the absence of a match in 5,000 men …show more content…
cules that replicate separately from the much larger bacterial chromosome * DNA containing the gene of interest is isolated * Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle * DNA with the target gene is treated with the same enzyme and many fragments are produced * Plasmid and target DNA are mixed and associate with each other * Same restriction enzyme * Both plasmid used as the vector and the interest * Will create the same sticky ends that reveal the sequence * Recombinant DNA molecules are produced when DNA ligase joins plasmid and target segments together * The recombinant DNa is taken up by a bacterial cell * The bacterial cell reproduces to form a clone of cells

* The cutting tools used are bacterial enzymes called restriction enzymes * Work by chopping up the foreign DNA, a process that restricts the ability of the invader to infect the bacterium * Restriction site is the DNA sequence recognized by a particular restriction enzyme * Once a restriction site is recognized, the restriction enzyme cuts both strands of the DNA at specific points within the sequence * Restriction enzymes cut DNA at specific sequences * Each enzyme binds to DNA at a different restriction site * Many restriction enzymes make staggered cuts that produce restriction fragments with single-stranded ends called “sticky

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