Final Plaque-Purification Lab Report

Lab number 11 is also titled “Final Plaque-Purification”. The objective for this lab is the same as lab number 9. The materials needed in this lab are a sterilized Agar plate, sterilized, 1 P20 Micropipette. P20 micropipette tips, 4.5 milliliters of Top Ager, 0.5 milliliters of M. smegmatis, 2 serological pipets, and 5.0 milliliters of Phage buffer. The protocol is to create agreed on the bottom of the Agar plate to look like a checkerboard. Flip it back over with a cover face in the ceiling. Extract 4.5 milliliters of Top Agar and place it into the 0.5 milliliters of M.smegmatis. Release the Top Agar. Withdrawal of the mix liquid with the 2 serological pipets, place it back into the tube, withdraw the mixed liquid again and place all of the mixed liquids into the Agar plate. Take 5.0 milliliters of Phage buffer before and place it into the negative control box. Take 5.0 microliters of 10-1 liquid into theMicrocentrifugeand put it into the tentative negative one box. Do the same procedure for …show more content…
The objective of this lab was to get a high concentration of virus with a good amount of concentration to isolate the DNA. The materials for this lab are: stands for oakridge tube and high-titer lysate conical tube, 10 milliliters uncontaminated High-Titer lysate, serological pipette, Oakridge tube, 40 microliters of nuclease, incubator, and 4 milliliters of phage buffer. The protocol for lab number 21 is to transfer 10 milliliters uncontaminated High-Titer lysate using serological pipette into the Oakridge tube. Place it into an incubator for half an hour after adding 40 microliters of nuclease, as well as inverting the nucleus and high-titer lysate. After 30 minutes of being inside the incubator, allow the mix to sit for one hour outside the incubator. 4 milliliters of phage buffer is to be added into the nuclease-lysate mixture and gently inverted. Place the mixture in the fridge when