Seminal Fluid

Front 1

What anatomical parts are involved in semen production?

Back 1

1) testes and epididymis

2) seminal vesicles

3) prostate

4) urethral glands

Front 2

Role of testes and epididymis

Back 2

testes make spermatozoa and they mature in the epididymis all under the influence of testosterone

Front 3

Role of seminal vesicles

Back 3

make 60% of seminal fluid, flavin protein gives seminal fluid iridescent look, fructose is main source of energy

Front 4

Role of the prostate

Back 4

makes 20% of seminal fluid, enzyme acid phosphatase helps liquefy SF, coag factors

Front 5

Why does it matter that acid phosphatase is not in other fluids?

Back 5

rape cases

Front 6

Role of urethral glands

Back 6

make 10% of seminal fluid

Front 7

How long before specimen collection do habits effect the specimen?

Back 7

3 months: fevers, hot tubs, infection, antibiotics

Front 8

How long must you abstain from ejaculation before specimen collection?

Back 8

2-7 days

Front 9

What should the specimen be collected in?

Back 9

sterile glass or plastic container

*not a condom, spermicidal agents

Front 10

Maximum time between collection and analysis

Back 10

<1 hour

Front 11

How must the specimen be kept before analysis?

Back 11

warm, should sit in a 35 C incubator for 1/2 hour before to liquefy

Front 12

Normal/abnormal appearance

Back 12

normal: milky white, viscous

abnormal: blood, yellow (urine: acidic, spermicidal)

Front 13

normal viscosity

Back 13

should expel from pipette in distinct drops, 0-4 categories

Front 14

normal volume

Back 14

should be between 2-6mL

Front 15

normal/abnormal pH

Back 15

normal: 7.2-8

abnormal: >8 bacterial infection, <7 hampers motility and metabolism

Front 16

normal motility and forward progression

Back 16

scan 25 fields on high power, 50% or more should be motile, rate 1+-4+ on speed (4 sec normal) and amount of forward progression, must be moving forward

Front 17

normal count

Back 17

dilute with NaOH and let sit 1/2 hour to stop motility, normal 20-120 million/ mm3

Front 18

normal morphology

Back 18

oval head with long tapering tail, 70% should be normal

Front 19

normal viability

Back 19

common stains are eosin and nigrosin, viable sperm are bluish white, dead are red, dead should equal amount of non-motile, at least 50% should be viable

Front 20

fructose levels

Back 20

need to be enough for sperm energy

Front 21

agglutinating bodies

Back 21

can be from male or female, mix sperm with female serum to determine if its the female

Front 22

culturing of sperm

Back 22

done to check for bacterial infections, STDs

Front 23

How are post-vasectomy specimens looked at?

Back 23

done 2 months after procedure, then after 1 month 3 times

*should be no sperm!