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37 Cards in this Set

  • Front
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Phases of quality assurance

Pre-analytical phase


Analytical phase


Post-analutical phase

Factors that alter test results that are introduced into the specimen before testing,including before and during collection, and during transport, processing, and storage.

Pre-analytical errors

Mixing tubes by inversion

3-10 inversions depending on the additive present,type of tubr and manufacturers

How tubes should be transported?

Transported STOPPER UP

Importance of STOPPER UP during transport

Reduce agitation


Aids in clot formation


Prevent contact of the tube contents with the tube stopper(aerosol formation)

How to transfer blood specimens?

Usr plastic bag w/ biohazard logo,liquid tigt closure, slip pocket for paperwork

How transfer non-blood specimens?

Leak proof containers

How to transfer pneumatic tube system?

Protected from shock,sealed in zipper type plastic bag to contain spills

How long is the allowed time for blood sample clotting

20-30 mins.

Allowed time of transport of blood specimen to lab?

Within 45 mins of collection

Once arrived in the lab, allowed time for serum separation from cell

Within 1hr collection

The maximum delivery time limit recommended by CLSI

Maximum 2hrs

Delivery time limit on off-site test: non-additive and SST tubes

Completely clotted before centrifugation

Delivery time limit on off-site test:


Heparin gel barrier tube/ PST's

Centrifuged right away

Delivery time limit on off-site test:


Hematology test tubes(EDTA)

Never centrifuged

Body temperature

36.4-37.6°c

Room temperature

15-30°c

Refrigerated temp.

2-10°c

Frozen temp.

-20°c below

More than 37°c is required during specimen transport

Use activated heel warmer

Sample specimens requiring body temperature during transport

Cold agglutinin


Cryofibrinogen


Cryoglobulins

Chilled specimen handling

Slows down metabollic process and protects analytes

Light sensitive specimen transport

Wrap in aluminum foil


Use amber colored microcollection containers

"central processing" ot triage

Received specimen


Prepare speciemn for testing


Identify specimen


Logged/ accesuined specimens


Sort by department and type of processing


Evaluates suitability for testing

Specimen rejection criteria

Inadequate, inaccurate,or missing speciemn identification


Underfilling of additive tubes


Hemolysis


Wrong tube


Outdated tube


Improper handling of specimen


Quantity not sufficient


Wrong collection time


Light exposure


Delay in testing


Delay or error in processing

Machine that spins blood and other specimens at a high number of revolutions per minute

Centrifuge

Cause the cells and plasma or serum in blood specimens to separate

RPM(revolution per minute)/ RCF( relative centripital force)

Common contaminants on specimen when stopper is removed

Sweat


Powder from gloves

Sweat

Increase chloride in sample

Balanced centrifuge operation

Equal size of tubes with equal volume of specimen

Unvalanced centrifugation

Break specimen tubes


Ruin specimen


Aerosol formation

Centrifuging PLASMA specimens

Blood collected in tubes with anticoagulant


Citrated tube and Heparinized tube and PST's

Centrifuging SERUM specimens

Blood must be completely clotted before centrifugation

Complate clotting of SST and tubes w/ cloy activators

30 mins

Complete clotting of thrombin tubes

5 mins

Incomplete clotting may result to?

Fibrin formation

What to use when transferrinf sample durinf aliquot preparation

Use disposable transfer pipette