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37 Cards in this Set
- Front
- Back
Phases of quality assurance |
Pre-analytical phase Analytical phase Post-analutical phase |
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Factors that alter test results that are introduced into the specimen before testing,including before and during collection, and during transport, processing, and storage. |
Pre-analytical errors |
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Mixing tubes by inversion |
3-10 inversions depending on the additive present,type of tubr and manufacturers |
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How tubes should be transported? |
Transported STOPPER UP |
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Importance of STOPPER UP during transport |
Reduce agitation Aids in clot formation Prevent contact of the tube contents with the tube stopper(aerosol formation) |
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How to transfer blood specimens? |
Usr plastic bag w/ biohazard logo,liquid tigt closure, slip pocket for paperwork |
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How transfer non-blood specimens? |
Leak proof containers |
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How to transfer pneumatic tube system? |
Protected from shock,sealed in zipper type plastic bag to contain spills |
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How long is the allowed time for blood sample clotting |
20-30 mins. |
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Allowed time of transport of blood specimen to lab? |
Within 45 mins of collection |
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Once arrived in the lab, allowed time for serum separation from cell |
Within 1hr collection |
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The maximum delivery time limit recommended by CLSI |
Maximum 2hrs |
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Delivery time limit on off-site test: non-additive and SST tubes |
Completely clotted before centrifugation |
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Delivery time limit on off-site test: Heparin gel barrier tube/ PST's |
Centrifuged right away |
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Delivery time limit on off-site test: Hematology test tubes(EDTA) |
Never centrifuged |
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Body temperature |
36.4-37.6°c |
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Room temperature |
15-30°c |
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Refrigerated temp. |
2-10°c |
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Frozen temp. |
-20°c below |
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More than 37°c is required during specimen transport |
Use activated heel warmer |
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Sample specimens requiring body temperature during transport |
Cold agglutinin Cryofibrinogen Cryoglobulins |
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Chilled specimen handling |
Slows down metabollic process and protects analytes |
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Light sensitive specimen transport |
Wrap in aluminum foil Use amber colored microcollection containers |
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"central processing" ot triage |
Received specimen Prepare speciemn for testing Identify specimen Logged/ accesuined specimens Sort by department and type of processing Evaluates suitability for testing |
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Specimen rejection criteria |
Inadequate, inaccurate,or missing speciemn identification Underfilling of additive tubes Hemolysis Wrong tube Outdated tube Improper handling of specimen Quantity not sufficient Wrong collection time Light exposure Delay in testing Delay or error in processing |
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Machine that spins blood and other specimens at a high number of revolutions per minute |
Centrifuge |
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Cause the cells and plasma or serum in blood specimens to separate |
RPM(revolution per minute)/ RCF( relative centripital force) |
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Common contaminants on specimen when stopper is removed |
Sweat Powder from gloves |
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Sweat |
Increase chloride in sample |
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Balanced centrifuge operation |
Equal size of tubes with equal volume of specimen |
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Unvalanced centrifugation |
Break specimen tubes Ruin specimen Aerosol formation |
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Centrifuging PLASMA specimens |
Blood collected in tubes with anticoagulant Citrated tube and Heparinized tube and PST's |
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Centrifuging SERUM specimens |
Blood must be completely clotted before centrifugation |
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Complate clotting of SST and tubes w/ cloy activators |
30 mins |
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Complete clotting of thrombin tubes |
5 mins |
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Incomplete clotting may result to? |
Fibrin formation |
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What to use when transferrinf sample durinf aliquot preparation |
Use disposable transfer pipette |