Microbiology Test 1 Part 2

Front 1

How should infectious medical waste be sterilized in an autoclave?

Back 1

processed for 30 to 60 minutes at 132 centigrade and 15 psi pressure

Front 2

Standard precautions in the microbiology laboratory apply to

Back 2

clinical specimens that contain blood or body fluids

Front 3

What do Biosafety Level 1 agents require?

Back 3

require only standard good laboratory technique.

Front 4

A cleaning solution used on the laboratory bench top to kill bacteria that are potentially harmful to humans would be called a(n):

Back 4

disinfectant.

Front 5

A cleaning solution used on the venipuncture site to avoid skin contamination of the blood sample is called a(n):

Back 5

antiseptic

Front 6

Which is the agency that has published guidelines known as standard precautions to reduce the risk of disease transmission in laboratories?

Back 6

CDC

Front 7

Class Ill biological safety cabinets are:

Back 7

are enclosed completely and have gloves that enable the worker to manipulate the material in the cabinet

Front 8

Biosafety Level 2 agents include:

Back 8

include those that are the common agents of infectious disease

Front 9

Biosafety Level 4 agents require:

Back 9

require the use of maximum containment facilities

Front 10

What is the principal role of the clinical microbiologist?

Back 10

d

Front 11

The definitive identification of clinically significant isolates is

Back 11

desirable in most cases but sometimes limited identification may be necessary and helpful.

Front 12

Holding media, such as Stuart's medium and Amie's transport medium, are used to

Back 12

maintain the viability of microorganisms present in a specimen

Front 13

What purpose does Direct examinations such as the Gram stain serve?

Back 13

To assess the quality of the specimen, to give the physician an early indication of what may be wrong with the patient, To guide the workup of the specimen

Front 14

Which type of media is designed to support the growth of a wide range of microorganisms?

Back 14

Nutritive

Front 15

Which type of media is designed to distinguish microorganisms on the basis of certain growth characteristics?

Back 15

Differential

Front 16

Which type of media is designed to support the growth of one group of organisms, but not another, by containing antimicrobials, dyes, or alcohol?

Back 16

Selective

Front 17

Examples of a selective medium for gram-positive organisms are:

Back 17

Colstin, nelidixic acid

Front 18

Which type of organism best suits the environmental condition of ambient air, which contains 21% oxygen (02) and a small amount (0.03%) of carbon dioxide (C02)?

Back 18

Aerobes

Front 19

Increased concentrations of C02 (5% to 10%) and approximately 15% 02 produce an environmental condition that best suits which type of organism?

Back 19

Capnophiles

Front 20

Reduced 02 (5% to 10%) and increased C02 (8% to 10%) produce an environmental condition that best suits which type of organism?

Back 20

Microaerophiles

Front 21

Which specimen is quantitatively plated by using a calibrated loop?

Back 21

Urine

Front 22

The first step in the Gram-stain sequence is the application¬¬¬¬¬¬_____ of which is the primary stain.

Back 22

crystal violet

Front 23

A mordant that is applied after the primary stain to bond chemically the alkaline dye to the bacterial cell wall is:

Back 23

Gram's iodine

Front 24

The acid-fast stain is specifically designed for bacteria whose cell walls contain:

Back 24

mycolic acids

Front 25

In the Kinyoun acid-fast staining method, ______ replaces ______ in the primary stain solution.

Back 25

increased phenol; heat

Front 26

What does the condenser do in dark field microscopy?

Back 26

This type of microscopy is used in some laboratories to detect spirochetes, the condenser does not allow light to pass directly through the specimen but directs the light to hit the specimen at an oblique angle, this technique involves the alteration of microscopic technique rather than the use of dyes or stains to achieve contrastdoes not allow light to pass directly through the specimen but directs the light to hit the specimen at an oblique angle,

Front 27

Magnifications in excess of 100,000 0 are achieved by______ microscopy?

Back 27

Electron

Front 28

The most critical step in the Gram stain is the application of the:

Back 28

Decolorizer

Front 29

A bacterial colony can be described as:

Back 29

v

Front 30

Which category of media can be described as containing specific nutrients required for the growth of particular bacterial pathogens in a patient specimen?

Back 30

Enrichment

Front 31

Which category of media can be described as containing some factor or factors that allow colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate?

Back 31

Differential

Front 32

Which organism is catalase positive, coagulase negative, pyrrolidonyi arylamidase (PYR) positive, susceptible to novobiocin, urease negative, and beta hemolytic on sheep blood agar?

Back 32

Staphylohemolytic