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33 Cards in this Set
- Front
- Back
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What is the highest total magnification that our microscopes can achieve? |
1500x |
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In microbiology, a negative staining technique is often used to help visualize bacteria or bacterial structures. For instance, capsules are often visualized by using a negative staining technique. In this context, what does the term 'negative' mean? |
That the bacterial cells are negatively charged, same as the negatively charged dye chromogen, making the bacteria not take up the dye repelling the chromogen dye, that way it facilitates the observation of bacterial morphology size and arrangement. |
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One of the stains we used this semester was Ethidium Bromide. What type of structure/structures was the stain used to visualize |
DNA |
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What is the purpose/ function of the "alcohol rinse" in the Gram Stain procedure? How long should you rinse with alcohol? |
The purpose is to decolorize the dye and you should only rinse for 5-10 seconds |
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Name the sugars present in TSI agar |
Glucose, Sucrose and Lactose |
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Triple Sugar Iron agar is used to identify bacteria capable of sulfur reduction? |
True |
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In microbiology, any nutritional substance used to grow bacteria is known as? |
Culture Media |
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What does it mean when a microorganism is ubiquitous? |
That they can be found in every habitat on earth |
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What is a culture media? |
Any nutrient source that allows microorganism to grow. |
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What two types of Culture media exist? |
Liquid, broth Solid, contains agar |
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What is a petri plate? |
It is a round plastic culture plate with loose-fitting lids |
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What is general purpose media? |
It is the media that allows the growth of many microorganisms that do not need special growth factors |
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What are some examples of general purpose media? |
Nutrient broth, tryptic soy broth, and tryptic soy agar |
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What are commensals? |
They are the microbes that inhabit our bodies in ways that may benefit both them and us |
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What is enrichment media? |
It contains special growth factors required by some microorganisms to grow |
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An example of enrichment media? |
Blood agar |
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What is selective media? |
It is used to inhibit the growth of some groups microorganisms and select for the growth of others |
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What is an example of selective media? |
Sabouraud's dextrose agar |
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What does SDA select for? |
It selects for fungi |
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What is differential media? |
It shows the differences between microbial colonies based on their cultural characteristics |
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What is differential media? |
It shows the differences between microbial colonies based on their cultural characteristics |
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What is an example of differential media? |
Blood agar |
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What is a colony? |
Clones from one bacterium |
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What is the optimal temperature for mold growth? |
25C |
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What is the optimum temperature for bacterial growth? |
35C-37C |
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What are the objective lens? |
4x Scanner 10x Low power 40x High dry 100x oil immersion |
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What are the objective lens? |
4x Scanner 10x Low power 40x High dry 100x oil immersion |
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What is the fiction of the iris diaphragm? |
To regulate the amount of light reaching the slide |
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What is the resolving power? |
The minimal distance two objects must be apart in order to distinguish them as separate and distinct |
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What is the resolving power? |
The minimal distance two objects must be apart in order to distinguish them as separate and distinct |
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What is the maximum resolution? And what does that mean? |
The maximum resolution is 0.2 micrometers. This means that objects on the slide which are less that 0.2 micrometers cannot be resolved at separate and distinct Objects |
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What is parfocal? |
It is the property of a microscope whereby the image will remain in focus when changing from low power lens to high power lens |
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What is the working distance? |
It is the distance between the objective lens and the slide when in focus. |
