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80 Cards in this Set

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percentage of persons without the disease who have a negative result

Clinical Specificity

True Negative = 90% Specificity


FP = False Positive (10%)

due to low molecular weight compounds that have contact with the skin

contact dermititis

most common causes of contact dermatitis include

1. nickel


2. rubber


3. poison ivy


4. cosmetics

causes sytemic inflammation due to infection of animal serum to humans

serum sickness

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

inactivation of serum

heating of serum @ 56 C for 30 mins

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

inactivation of serum

heating of serum @ 56 C for 30 mins

reinactivation of serum (when 4 hrs has lapsed)

heating of serum at 56 C for 10 mins

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

inactivation of serum

heating of serum @ 56 C for 30 mins

reinactivation of serum (when 4 hrs has lapsed)

heating of serum at 56 C for 10 mins

requires the use of serum

1. VDRL


2. FTA-ABS


3. Complement Fixation Test

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

if this substance is present in the reagent there is no need to heat sserum

choline chloride

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

inactivation of serum

heating of serum @ 56 C for 30 mins

reinactivation of serum (when 4 hrs has lapsed)

heating of serum at 56 C for 10 mins

requires the use of serum

1. VDRL


2. FTA-ABS


3. Complement Fixation Test

based on the principle that soluble antigens from Mycobacterium tuberculosis induce a reaction in people who have past or present infection of tuberculosis

Tuberculin test

if this substance is present in the reagent there is no need to heat sserum

choline chloride

represent the ratio of concentrated or stock material to the total final volume of a solution

Dilution

other name of tuberculin test

PPD - purified protein derivative/ mantoux test

what is injected under the skin in mantoux test and read at what hours?

PPD; read at 48-72 hours

positive result for mantoux test

(+) erythema/induration

commonly used sample in serology

serum

blood is allowed to clot at what temp?

room temp or 4 C then centrifuged

if testing cannot be performed immediately:

Ref temp:


< 72 hrs serum is stored @ 2-8 C


Freezing temp:


> 72 hrs serum is stored at -20 C

inactivation of serum

heating of serum @ 56 C for 30 mins

reinactivation of serum (when 4 hrs has lapsed)

heating of serum at 56 C for 10 mins

requires the use of serum

1. VDRL


2. FTA-ABS


3. Complement Fixation Test

dilution is an indication of …

relative concentration

dilution is an indication of …

relative concentration

dilution can be expressed as…

fraction or ratio

Types of dilution

1. simple


2. compound


3. serial dilution