Herpesviruses

Front 1

The subdivision of the family herpeviridae is primarily based on?

Back 1

Cell tropism

Genome organization

Front 2

What are the 3 subfamilies and what are their characteristics?

Back 2

Alpha: short growth cycle, rapid spread, latent in sensory neurons

Beta: long growth cycle, slow spread, restricted host range. Latent in secretory glands and lmphoreticular cells

Gamma: growth primarily in epithelial cells and B or T lymphocytes. Latent in B cells

Front 3

Herpesvirus infection typically involve?

Back 3

Acute replication followed by the establishment of lifelong latency

Front 4

Lytic Infection:

Back 4

Thousands of new infectious viral particles produced

60-70 viral genes expressed

Front 5

During latency, the herpesvirus genome is maintained as:

Back 5

An episome

Front 6

HSV-1 virus particles consist of?

Back 6

Envelope spiked w/10 different virus glycoproteins

Tegument w/14 free proteins

Icosahedral capsid made of 6 virion proteins

Linear dsDNA

Front 7

Tegument proteins are released ________ entry and are crucial for establishing infection

Back 7

After

Front 8

Although herpesvirus genome structures are variable, most ________ genes are cell conserved amoung herpesviruses.

Back 8

DNA replication

Front 9

Herpesvirus tegument proteins play an important role in early stages of infection because they:

Back 9

Are real eased into the cytoplasm

Front 10

List the steps involved with entry for HSV-1.

Back 10

1. Entry is mediated by envelope glycoproteins (gB and gC bind to heparin sulfate)

2. Binding triggers fusion of the virus envelope w/cellular membrane

3. Tegument proteins and capsid are released into the cytoplasm

4. Capsids detach from tegument and move toward nucleus

5. Capsid bind at nuclear envelope and release DNA into nucleus

Front 11

Herpesvirus lytic gene expression follows a highly orders cascade in which the protein products of early genes?

Back 11

Facilitate viral DNA replication and transactivate the expression of late genes

Front 12

List the steps that occur in HSV-1 rolling circle replication:

Back 12

1. One of parental DNA strands nicked, leading strand is pulled away from inner parent molecule

2. Additional DNA polymerase and process invite factor synthesize the complementar lagging strand

3. Generates a linear, dsDNA head to tail concatamer

Front 13

List the steps in HSV-1 assembly:

Back 13

1. Immature capsid is assembled by accumulation of VPS and other proteins around a scaffold

2. Virus DNA entry into capsid is triggered when packaging machiner encounter packaging sites within the alpha segment of the genome

3. DNA packaging is complete when a full length genome is inserted and the machinery encounters another terminal alpha segment

4. Upon termination the scaffolding is dismantled and the scaffold proteins are ejected from capsid

5. Capsid changes conformation sealing the DNA inside the capsid

Front 14

What are the 4 characteristics of latency?

Back 14

1. Non-productive infection

2. Maintenance of the viral genome as an episome

3. Few or no virus genes expressed

4. The ability to deactivate from latency

Front 15

When herpesvirus enters local sensory neurons that innervate that site they lead the Nucleocapsid along the axon in ___________ transport

Back 15

Retrograde

Front 16

What are the two most common types of HSV and what type of infections are they usually associated with?

Back 16

HSV-1: oral herpetic lesions (can also cause herpes storm all keratitis of the eye leads to blindness)

HSV-2: genital herpetic lesions

Front 17

Alpha HVs usually cause?

Back 17

Chickenpox

Shingles

HKS

Oral herpetic lesions

Genital herpetic lesions

Encephalitis

Front 18

What is the gamma herpesvirus that establishes life-long latency in B cells?

Back 18

Epstein Barr Virus (EBV)