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6 Cards in this Set
- Front
- Back
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Describe three problems associated with using the standard plate count method for determining the number of bacteria in a sample. |
1. Each colony may represent more than one bacterial cell 2. Medium used may affect what type of bacteria is able to grow 3. Number represents only the living cells, not the total number of cells |
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What are the units used for reported the number of cells determined by a standard plate count? |
Colony-forming units |
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When using the standard plate count, only numbers between ....... and ....... are considered statistically valid? |
30 and 300 |
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Describe two things you should never do with pipettes? |
1. Never touch tip or barrel of pipettes as it can lead to contamination 2. Contaminate other pipettes with your hands when taking a pipette out from the canister |
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You are asked to add 2 mL of bacterial culture to 98 mL of water. By what factor are you diluting the bacterial culture? |
1:50 |
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You are asked to make a 1000 X (or 1:1000)dilution of a bacterial culture in a final volume of 5000 mL. What volume ofthe original culture would you use? Include the units.
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5 mL |
