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247 Cards in this Set

  • Front
  • Back

Where does the krebs cycle take place?

Takes place within mitochondrial matrix

What must happen before the citric acid cycle can begin?

Pyruvate needs to be converted to Acetyl-CoA (before what?)What

What molecules are generated for one turn of the citric acid cycle?

1 ATP + 3 NADH + 1 FADH2 generated (per one turn if what?)

How many turns of the citric acid cycle per glucose?

2 turns

Coenzyme A (What does it do?)

Converts pyruvate to Acetyl CoA (what does?)

Citrate Synthase (what reaction does it catalyze)

Acetyl CoA adds to 2C to oxaloacetate (which enzyme)

CoA - SH (when is it released?)

Step 1 of citric acid cycle ( What is it released?)

Aconitase (What does it catalyze?)

Citrate to Isocitrate {isomers} (Catalyzed by what enzyme?)

Isocitrate dehydrogenase (what reaction does it catalyze?)

Isocitrate oxidized


NAD + reduced to NADH (catalyzed by what?

a-ketglutarate (product of what?)

Step 3 of calvin cycle (produces what?)

a-ketoglutarate dehydrogenase (catalyzes what?)

a-ketogkutarate loses 1 CO2, NAD+ reduced to NADH, remaining molecues attached to CoA-SH (catalyzed by what?)

succinyl-CoA synthetase (catalyzes what?)

CoA displaced by PO4, PO4 transferred to GDP, GDP -> GTP -> transfers PO4 to ADP = ATP (catalyzed by what?)

Succinate (formed when/where)

Step 5 of Citric Acid cycle (generates what?)

Succinic dehydrogenase (Catalyzes what?

Succinate oxidized : 2H to FAD = FADH2 {fumarate} (catalyzed by what?)

FAD (what does it stand for?)

Flavin Adenin Dinucleotide (Acronym?)

Fumarase (catalyzes what?)

Fumarate + H20 = malate

Malate Dehydrogenase (Catalyzes what?)

Malate oxidized, NAD+ -> NADH = oxaloacetate (catalyzed by what)

NAD+ reduced to NADH (& an H+) + 1 CO2 lost (what step of krebs cycle)

Step 3 (What happens)

NAD+ reduced to NADH & H+, CO2 released, CoA-SH used (What step of Krebs cycle?)

Step 4 (what intermediaries are released/transformed/used in this step of the krebs cycle)

CoA-SH produced, Phosphate group used (What step of the krebs cycle)

Step 5 of the Krebs cycle (what intermediaries are produced/released)

How many CO2 molecules are generated per turn of the citric acid cycle

2 CO2 molecules (generated per one turn of what cycle?)

Oxidoreductases (What do they do?)

Catalyzes transfer of electrons from one molecule (What type of enzyme is this?)

Transferases (What do they do?)

Enact the transfer of specific functional groups from one molecule (What Enzyme is this?)

Hydrolases (What do they do?)

Catalyses hydrolysis of a substrate (What Enzyme does this?)

Lyases (What do they do?)

Catalyses breaking of various chemical bonds by means other than hydrolysis or oxidation (What Enzyme is this?)

Isomerases (What do they do?)

Catalyzes conversion of a compound to an isomer (What Enzyme is this?)

Ligases (What do they do?)

Catalyze joining of two large molecules by forming a new bond, usually accompanying hydrolysis (What enzyme does this?)

Kinases (What do they do?)

Catalyzes transfer of phosphate groups from high-energy phosphate-donating molecules to specific substrates (What enzyme does this?)

Dehydrogenases (What do they do?)

Catalyze removal of hydrogen atoms from a molecule (What enzyme does this?)

In what form is the energy extracted from food found as?

ATP -> directly available


NADH//FADH2 -> electron carriers, contains most of the energy


(What role do these molecules have? What do they store?)

NADH//FADH2 (What is their role?)

Donate electrons to ETC, powers ATP synthesis via oxidative phosphorylation

Where is the ETC located?

Inner membrane (cristae) of mitochondrion (what is located here?)

What are the components of the ETC?

Proteins arranged in multiprotein complexes I-IV, Cytochromes, Ubiquinone (Components of what?)

What is the function of the ETC?

Break large free energy drop from food to O2 into smaller steps that release energy in manageable amounts (Function of what?)

Chemiosmosis (What is it?)

Use of energy in H+ gradient to drive cellular work (What is the name for this?)

How much ATP can be made with 1 NADH

2.5 ATP (Generated with what molecule?)

How much ATP can be made with one FADH2

1.5 ATP (Generated with what molecule?)

Fats digested as glycerol are used for what part of cell. respiration?

Glycolysis

What do fats digested as fatty acids generate through beta-oxidation?

Acetyl CoA (Generated by what molecules through beta-oxidation?)

How much ATP does an oxidized gram of fat yield?

2 ATP (generate by an oxidized gram of what?)

What does one Beta-Oxidation cycle generate?

1 NADH, 1 FADH2 & 1 acetyl CoA (generated by one cycle of which process?) `

How much ATP does the oxidation of ATP yield?

16.5 to 18.5 ATP (Generated by which process?)

Which amino acids are not glucogenic?

Leucine & Lysine (What characterizes these two amino acids?)

What are the two Ketone bodies that are converted to Acetyl-CoA & used as a source of energy for brain&heart

Acetoacetic acid and beta-hydrobutyric acid (used for what?)

What are the two things that inhibit phosphofructokinase?

ATP and Citrate (Inhibit what?)

What stimulates phosphofructokinase?

AMP concentration

What happens during periods of low energy demand?

Excess ATP used to convert creatine to phosphocreatine (Happens when?)

What happens during periods of high energy demand?

Phospocreatine anaerobically donates a phosphate group to ADP to form ATP (Happens when?)

How does the Phosphagen system generate ATP?

Creatine Kinase transfers P from creatine phosphate to ADP (What energy system is this?)

What are a few alternatives to O2 as final electron acceptor?

Sulfur, Nitrate, CO2, inorganic metals (used as what in prokaryotes?)

Methanogens (what do they do?)

Create Methane, CO2 reduced to CH4 (Who does this?)

Sulfur bacteria (What do they do?)

SO4 is reduced to hydrogen sulfide (H2) (Who does this?)

Sulfate-reducing bacteria (What can they do?)

Anaerobically oxidize methane formed by methanogens, oxidize organic compounds or molecular hydrogen while reducing sulfate to hydrogen sulfide (Who does this?)

Alcohol Fermentation (What Happens?)

Pyruvate converted to Ethanol in two steps, CO2 and NAD+ produced (What process is this?)

Lactic Acid Fermentation (What happens?)

Pyruvate reduced by NADH, lactate formed (ionized lactic acid)

How much ATP per glucose does lactic acid fermentation produce?

2 ATP/Glucose (what process produces this many ATP/Glucose)

Converts lactate to pyruvate

Lactate Dehydrogenase (converts what to what?)

Final electron acceptors in fermentation?

Pyruvate or Acetaldehyde (what role in fermentation?)

What organism is responsible for most of the photosynthetic activity on earth?

Unicellular Algae (responsible for what?)

Purple Sulfur Bacteria (What are they?)

Anaerobic/Photosynthetic bacteria (What are they called?)

Where are chloroplasts found?

Mesophyl cells in interior tissue of the leaf (What is found here?)

How many Chloroplasts are found in a Mesophyl?

30-40 Chloroplasts (found where?)

Stromata (What are they?)

Pores in leaf (What are they called?)

Why is photosynthesis a redox process?

H20 is oxidized & CO2 is reduced (Describes what process?)

What happens during the light reactions of photosynthesis?

light energy transformed in Chemical energy (Occurs in which part of photosynthesis?)

What are the results of the light reactions?

H20 split, O2 released, NADP+ to NADPH, ATP from ADP (results of what part of photosynthesis?)

What are the results of the Calvin Cycle?

Sugar formed, CO2 formed, ATP&NADPH used (results of what part of photosynthesis?)

What does the Calvin Cycle begin with?

Carbon fixation (beginning of which part of Photosynthesis?)

Accessory Pigments (What are they called?)

Carotenoids, Chlorophyl II b (What are these?)

Action Spectrum (What is it?)

Relative Effectiveness of different wavelengths in driving photoshyntesis (What is the word for this?)

Photosystem I (what wavelength?)

700nm (Which Photosystem has this wavelength?)

Photosystem II (What wavelength?)

680nm (Which Photosystem has this wavelength?)

Photosystem (What is it?)

reaction-center complexes (protein complex) surrounded by light harvesting complexes (What is this called?)

Light harvesting complexes (What are they?)

Pigment molecules bound to proteins (What is the word for this?)

Light harvesting complexes (What do they do?)

Funnel energy of photons to reaction center (What complexes fulfill this role?)

What is the first step of the light reactions?

Solar-Powered transfer of electrons from chlorophyll a to primary electron acceptor (What step of which part of photosynthesis is this?)

Which Photosystem happens first?

Photosystem II (happens first or second?)

What are the two possible routes for electron flow during the light reactions?

Linear Electron flow & Cyclic Electron flow



What is the primary pathway that involves both photosystems and produces ATP//NADPH?

Linear Electron flow (what characterizes this pathway?)

Linear Electron flow step 1 (What happens?)

Photon hits a pigment in light-harvesting complex of Ps II, energy passes among pigment molecules until it excites P680 pair of chlorophyll a

Linear Electron flow step 2 (What happens?)

excited electron from P680 is transferred to primary electron acceptor (Phaephytin) and P680 becomes P680+



Phaephytin (What is it?)

Chlorphyll a without Mg 2+ (What is it?)

Linear Electron flow step 3(What happens?)

Enzyme catalyzes splitting of water, e transferred one by one to P680+ , two Os combine, 2 H+ released

Linear Electron flow step 4 (What happens?)

Photoexited electron passes from primary electron acceptor of PSII to PSI via electron transport chain

What is the ETC in linear electron flow made of

Plastoquinone, Cytochrome Complex, Plastocyanin

Linear Electron flow step 5 (What Happens?)

Fall of electron is used to pump protons across thylakoid membrane, used in Chemiosmosis

Linear Electron flow step 6 (What Happens?)

Process from PS2 repeated in PS1, energy passed among pigment molecules until it excites P700

Linear Electron flow step 7 (What Happens?)

Photoexcited electron passes from primary electron acceptor of PSI down a second electron transport chain through ferredoxin (Fd), does not create proton gradient

Linear Electron flow step 8 (What Happens?)

NADP reductase transfers electrons from Fd to NADP+ = NADPH, used in calvin cycle

What photosystem is used by cyclic electron flow?

only photosystem I used by cyclic electron flow

What is produced and what is not produced by cyclic electron flow?

ATP produced, NADH and Oxygen not produced

What might cyclic electron flow protect cells from?

Light-induced damage (What protects plants from this?)

Role of Plastoquinone in chemiosmosis in chloroplasts?

Transfers electron to cytochrome complex

What powers ATP synthase in Chloroplasts?

Diffusion of H+ from thylakoid space to stroma (down concentration gradient)_

What does the Calvin Cycle use to convert CO2 to sugar

ATP & NADPH (used to do what in the calvin cycle?)

What does the calvin cycle use to build G3P

Smaller molecules using ATP & reducing power of electrons carried by NADPH (What is built using this?)

How many times must the carbon cycle take place for 1 G3P to be produced

Three times (What cycle must take place this many times for 1 G3P to be produced?)

How much CO2 molecules need to be fixed by the calvin cycle in order for one G3P to be produced?

3 CO2 molecules (produced by 3 turns of what cycle?)

3 phases of Calvin Cycle?

Carbon Fixation, Reduction, Regeneration of RuBP (CO2 acceptor) (3 phases of which cycle?)

RUBISCO (What does it stand for?)

Ribulose Bisphosphate Carboxylase Oxidase

Result of CO2 being attached to RuBP

Short-lived GC intermediate followed by 3-phosphoglycerate (Result of What?)

Result of phosphorylation of 3-phosphoglycerate by ATP

1-3 bisphosphoglycerate (result of what?)

Result of reduction of 1-3 bisphosphoglycerate by NADPH

ATP (result of reduction of what molecule by NADPH in calvin cycle?)

Product of re-arrangement of Carbon skeleton of 5 G3P

3 RuBP (15 carbon total) (Product of what?)

Required for the synthesis of 1 G3P by Calvin Cycle

3x cycle, 9ATP, 6NADPH (required for synthesis of what?)

two enzymatic activities of Rubisco

Carboxylation and Photorespiration (Enzymatic activies of which enzyme?)

Carboxylation (Favored When?)

Favored under normal conditions (Which enzymatic activity is?)

Photorespiration (Favored When?)

Favoured when stoma are closed in hot conditions

Advantages/Disadvantages of Photorespiration?

allows nitrate assimilation from soil, reduces free radicals, can drain as much as 50% of carbon fixed by calvin cycle

Photorespiration vs. Carboxylation?

O2 added to RuBP instead of CO2, O2 consumed & CO2 released without producing ATP/sugar

How much does Photorespiration cost?

1 NADPH + 1 ATP/Cycle (Cost of what process?)

C3 carbon fixation (what is it?)

fix carbon using only C3 photosynthesis (Calvin Cycle) (What is the name for this?)

How does C4 carbon fixation minimize the cost of photorespiration?

By incorporating CO2 in 4 carbon cmpds in mesophyll cells (Which type of carbon fixation accomplishes what?)

Where are the 4-carbon compounds from C4 fixation exported

Bundle-Sheath cells (What compound from C4 fixation is exported here?)

Which enzyme does C4 fixation require?

PEP Carboxylase (required by what kind of carbon fixation?)

How many extra ATPs are required by C4 fixation as compared to the carbon cycle?

12 extra ATPs required (required by what kind of carbon fixation?)

Type of separation for CAM plants?

Temporal Separation (separation for which type of plants?)

How many H+ through membrane with NADH?

10 H+

How many H+ through membrane with FADH2

6 H+

How much H+ through ATP synthase for 1 ATP?

4H+

What is Metabolism?

The total sum of annabolic and Catabolic reactions

What are the two types of Metabolic Pathways?

Catabolic & Anabolic Pathways

Catabolic Pathways (what are they?)

Release energy, breakdown complex molecules

Catabolic Pathway (example)

Cellular Respiration

Annabolic Pathway (what is it?)

Consumes energy to build complex molecule from simpler ones

Annabolic Pathway (example?)
synthesis of proteins

First law of thermodynamics (what is it?)

Energy of the universe is constant

2nd law of thermodynamics (what is it?)

In every energy transfer or transformation, some energy is unusable

In what case do chemical reactions occur spontaneously?

When they increase the entropy of the universe

How can entropy in an organism be increased?

More complex structures can be created

What is energy?
The capacity to cause change

What is a living system's free energy?

Energy that can do work when pressure and temperature are uniform

Which processes are spontaneous?

Processes with a negative change in free energy

Endergonic (characteristics?)

Products have more free energy than reactants, Not spontaneous

Exergonic (characteristics?)

Products have less free energy than reactants, spontaneous

How does the amount of free energy affect stability?

More free energy = less stable, less free energy = more stable

Energy Coupling (what is it?)

Use of an exergonic process to drive an endergonic one

Mediates energy coupling in cells
ATP

What is ATP made up of?

Ribose sugar, Adenine nitrogenous base, 3 phosphate groups

Is ATP hydrolysis exergonic or endergonic

exergonic

How does ATP drive endergonic rxn?

by phosphorylation

What is phosphorylation?

Transfer of a phosphate group to some other molecule

How can the active site of an enzyme lower an EA barrier?

By orienting substrate correctly, straining substrate bonds, providing favorable microenvironment, and covalently bonding to the substrate

Apoenzyme (what is it?)

Protein Portion, inactive

Holoenzyme (what is it?)

Whole Enzyme (active)

Why is cellular respiration a redox process?

Hydrogen with electrons is transferred from glucose to oxygen , energy released

Essentially, what is cellular respiration?

Gradual oxidization of glucose

NAD+ (what does it do?)

Harnesses potential energy by capturing electrons

What does NAD+ become after it captures electrons?

NADH

NADH (what does it do?)

transfers electrons to ETC

Where does glycolysis take place?

In the cytoplasm

What process forms most of the ATP?

Oxidative phosphorylation

Substrate-level phosphorylation (what does it do?)

forms small amount of ATP in glycolysis and citric acid cycle

Glycolysis (what are the two major phases?)

Energy investment phase & energy payoff phase

Step 1 (EIP)

Glucose enters cell, Glucose-6-phosphate produced

Step 1 (EIP) [what enzyme?]

Hexokinase

Step 1 (EIP) [what is invested?]

1 ATP

Step 2 (EIP)

Glucose-6-phosphate to fructose-6-phosphate

Step 2 (EIP) [What enzyme]

Phosphoglucoisomerase

Step 3 (EIP)

Fructose-6-phosphate phosphorylated to fructose 1,6-bisphosphate

Step 3 (EIP) [What enzyme?]

Phosphofructokinase

Step 3 (EIP) [What is invested?]

1 ATP

How is phosphofructokinase regulated

regulated allosterically by ATP & its products
Step 4 (EIP)

Fructose 1,6-bisphosphate cleaved to dihydroxyacetone phosphate + glyceraldehyde-3-phosphate

Step 4 (EIP) [what enzyme?]

Aldolase

Step 5 (EIP)

Conversion between G3P and Dihydroxyacetone phosphate

Step 5 (EIP) [What enzyme?]

Isomerase

Step 5 (EIP) [In which direction is the reaction pulled?]

Reaction pulled in direction of G3P

Energy Payoff phase (what is the net product?)

2 G3P

Step 6 (EPP)

G3P to 1,3-bisphosphoglycerate

Step 6 (EPP) [What are the two reactions]

G3P oxidized (e/H+ transferred NAD+ -> NADH), Energy released to attach phosphate to oxidized substrate

Step 6 (EPP) [What enzyme?]

Triose phosphate dehydrogenase

Step 6 (EPP) [transfer of e/H+ to NAD+ exergonic or endergonic?]

Exergonic

Step 6 (EPP) [Intermediaries?)

2 NAD+ to 2NADH + 2H+ & 2 phosphate groups release

Step 7 (EPP)

1,3-Bisphosphoglycerate to 3-phosphoglycerate

Step 7 (EPP) [What Enzyme?]

Phosphoglycero-kinase

Step 7 (EPP) [Intermediaries]

2 ATP produced, (2 ATP/glucose invested)

What reaction involving ATP happens in Step 7 of glycolysis

Exergonic reaction; PO4 added to ADP to make ATP

Where does the energy for adding PO4 to ADP come from

Energy comes from oxidation of sugar in Step 6

Step 8 (EPP)

PO4 relocated on 3-phosphoglycerate to obtain 2-phosphoglycerate

Step 8 (EPP) [What enzyme?]

Phosphoglyceromutase

Step 9 (EPP)

Double bond formed in 2-phosphoglycerate to form PEP & H2O

Step 9 (EPP) [What enzyme?]

Enolase

Step 10 (EPP)

PEP to Pyruvate

Step 10 (EPP) [What enzyme?]

Pyruvate Kinase

Step 10 (EPP) [Intermediaries?]

PO4 transferred from PEP to ADP -> ATP

Net yield of glycolysis?

2 ATP/Glucose + 2 NADH/glucose

What will the pyruvate produced in glycolysis be used for?

Citric acid cycle (if oxygen present) or fermentation (if oxygen not present)

When does Substrate-level phosphorylation happen in Glycolysis

Steps 7&10

Phosphorylated by Hexokinase

Glucose

converted to isomer by phosphogluco-isomerase

Glucose-6-phosphate

Phosphorylated by phosphofructokinase

Fructose-6-phosphated

Cleaved by Aldolase

Fructose-1,6-bisphosphate

Isomerase catalyzes conversion between which two isomers?

G3P & Dihydroxyacetone phosphate

Converted to 1,3-Bisphosphoglycerate by Triose Phosphate Dehydrogenase

G3P

Converted to 3-Phosphoglycerate by Phosphoglycerokinase

1,3-Bisphosphoglycerate

Converted to 2-Phosphoglycerate by Phosphoglyceromutase

3-Phosphoglycerate

Converted to PEP by Enolase

2-Phosphoglycerate

Converted to Pyruvate by Pyruvate Kinase

PEP

At what step of Glycolysis is H2O released?

Step 9

Why do animals store most of their energy as fats?

Oxidized gram of fat produces twice as much ATP as an oxidized gram of carbohydrate

What would an 18C fatty acid chain going through beta-oxidation produce?

8NADH, 8FADH2, 9 acetyl CoA

Oxidation of glycerol (what is invested/what is produced?)

1 ATP invested, 1 NADH produced

What is formed when FAs are broken down in liver

ketone bodies

Thylakoid Stacks (what are they called?)

Grana

Fluid in chloroplasts (what is it called?)

Stroma

Why is photosynthesis a redox process

H2O is oxidized and CO2 reduced

What are the two stages of photosynthesis

light reactions (photo), Calvin Cycle (synthesis)

Action Spectrum (what is it?)

Profiles relative effectiveness of different wavelengths in driving photosynthesis

What light works best for photosynthesis?

Violet-blue and red light

When was the action spectrum of photosynthesis first demonstrated?

In Engelmann's experiment

How was the action spectrum of photosynthesis first demonstrated?

Exposing algae to different wavelengths , areas receiving wavelengths favorable to photosynthesis produce more O2

What do sulfate-reducing bacteria do?

Oxidize organic compounds or molecular hydrogen while reducing sulfate to hydrogen sulfide

Sulfate reduction/Methane oxidation formula

CH4 + SO4^2- -> HCO3- + HS- + H2O

Basic fermentation equation

Organic molecule + NADH -> reduced organic molecule + NAD+

What is acetaldehyde's role in alcohol fermentation?

Accepts pair of electrons from NADH producing NAD+ and ethanol

Malate-aspartate shuttle (what is its role?)

NADH generated by fermentation enters matrix through this

What powers ATP synthase in chloroplasts?

Diffusion of H+ from thylakoid space to stroma (down concentration gradient)

Chemiosmosis in Mitochondria (source of energy?)

Chemical energy from food to ATP
Chemiosmosis in Chloroplasts (source of energy?)

Light energy into chemical energy of ATP

Spatial organization of chemiosmosis in Mitochondria

Protons pumped to intermembrane space & diffuse back into mitochondrial matrix

What type of pathway is the calvin cycle?

Anabolic pathway

In the calvin cycle, what precedes 3-phosphoglycerate?

6-Carbon intermediate

What is the most abundant protein on earth?

Rubisco

Hexose Isomerase (what does it do?)

F6P -> G6P

How much G3P is needed to make one F6P

2 G3P

Product of attachment of CO2 to RuBP by Rubisco (calvin cycle)

3-phosphoglycerate

Product of phosphorylation of 3-phosphoglycerate by ATP (calvin cycle)

1,3-bisphosphoglycerate

What happens when 1,3-bisphosphoglycerate is reduced by NADPH in the calvin cycle

PO4 lost, 6 G3P produced, 1 leaves cell, 5 recycled to generate 3 RuBP

How many ATP are dephosphorylated to ADP to re-arrange the carbon skeleton of 5G3P into 3 RuBP

3 ATP -> 3 ADP

What is the role of a P680 pair of chlorophyll a in linear electron flow

P680 becomes P680+ (gives off electron ) and splits H2O

In linear electron flow, what happens after H2O is split

O combines with O from other splitting to form O2, 2H+ released

In liner electron flow, where does the energy to pump protons across the Thylakoid membrane come from

Fall of é from PS II to PS I via ETC

After the splitting of H2O in Linear electron flow, what is the 2H+ released used for?

Contributes to proton-motive force

What is the role of P700 in Photosystem I

Becomes P700+, accepts é from ETC

In linear electron flow, what happens after electrons from the ETC are accepted by P700+

Pass from primary é acceptor down second transport chain through ferredoxin

At the end of linear electron flow, how is NADPH generated?

NADP+ reductase transfers electrons from Ferredoxin to NADPH

What conditions are created by closed Stoma in plants?

Low CO2 and high O2

What can decrease photorespiration rates?

Increased atmospheric CO2 concentrations

Why does photorespiration cost less in C4 plants?

PEP has a higher affinity for CO2 than Rubisco

How is CO2 incorporated into 4 carbon compounds in C4 plants?

PEP + CO2 -> Oxaloacetate (4C)

2 examples of C4 plants

Corn and sugarcane

Example of CAM plant

Pineapple

When do CAM plants open their stomata?

At night

What is O2 incorporated into in CAM plants?

organic acids

Gross Primary Productivity (what is it?)

Amount of Carbon fixed per unit of time in a given area

Net Primary Productivity (what is it?)

GPP - amount of carbon used in cell. resp. by photosynthetic organisms