Essay Isolation of Fungal Strains: An Experimental Study
Endophytic fungi are natural products which have specific ability to produce compounds having strong biological activities. Research on isolation of secondary metabolites from endophytic fungi of selected plants begin with chemical profiles revealed of secondary metabolites of its species. This fact became strong theoretical foundation to build a method of selecting bioactive compounds and extend its utilization and synthesis. Initial steps involved are selection, screening, dereplication, isolation, and structure elucidation process using spectroscopic methods combined with chemoinformatic analysis (Butler 2004; Larsen et al., 2005; Hassan 2007).
As part of a phytochemical study of our research, we have previously reported two alkaloid compounds which is active as antimalarial from endophytic fungus which was isolated from the stem and leave of brotowali (Elfita, 2011a). The purpose of the present study was to isolate and determine two lactones from Trichoderma sp. from roots of brotowali to get the profile of organic compounds produced by endophytic fungi of brotowali.
MATERIALS AND METHODS
The root of brotowali were collected on April 2012 from