What Are Hepg2 Cells ( American Type Culture Collection ), Manassas, Usa
2234 Words Sep 21st, 2016 9 Pages
Stock solutions of PAHs (B[a]P, Nap, Phe and Pyr) and metals (As, Cd and Pb) were prepared in DMSO and MilliQ water (18 MΩ.cm, (Merck Millipore, VIC, Australia), respectively. The working solutions prepared in DMEM + FBS (10%) medium or DMEM alone were used for MN test and Ahr CAFLUX assay, respectively. The working solutions were added to the plates reaching a final concentration of 0.5% v/v of vehicle control (DMSO or MilliQ water).
In vitro flow cytometry micronucleus test
The selected concentrations of individual MN study were 0 to 2 μM for B[a]P and Cd; 0 to 10 μM for As and 0 to 100 μM for Pb. Initially, the cytotoxicity of PAHs and metals were determined using the MTS assay. Based on their cytotoxicity to HepG2 cells, the concentrations of metals and PAHs were selected for the MN study. The treatment was carried out for 1.5 – 2…