The Regeneration Of Enzymatic Cofactors Essay

1885 Words Oct 17th, 2016 8 Pages
Regeneration of enzymatic cofactors is the most limiting technical challenge of employing biochemical synthetic reactions for industrial chemical manufacturing. Consequently, requirement and the high cost of these cofactors has limited, isolated biocatalysts for production of fine chemicals. We strived to engineer a drop-in, cofactor regeneration technology, circumventing limited use of isolated biocatalyst for consumer chemicals. Presented here is a biologically engineered artificial organelle that allows for proficient and environmental affable reduction of nicotinamide coenzymes; using light and sourcing electrons from water, generating oxygen as the sole by-product. This platform relies on the elegant nexus between two complex membrane proteins; Photosystem II with Respiratory Complex I function cooperatively to yield NAD+ reduction rates of 354.85 38.71 nmol min-1 mg CMI-1. The proposed technology could be easily used for any isolated enzyme process, which requires NADH recycling allowing wide adoption of biochemical synthetic methods for next generation chemical manufacturing.
Nicotinamide coenzymes (NAD(P)+/NAD(P)H) are the most frequently used cofactors for consumer chemicals manufactured using biocatalysts(1). Commonly, the cost of cofactors significantly exceeds the product value. Consequently, the requirement and the high cost of these cofactors has been prohibitive for industrialization of valuable redox enzymatic reactions. To make an industrial biosynthesis…

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