The Importance Of Neoplasm

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As aware of by most, one of the leading diseases that is causing a threat to our society currently is cancer. The prevalence of this disease is overwhelming and the treatment is possible; however, there is still need for further investigation into the cause of this disease before ¬¬¬¬¬it is thoroughly understood. Through increasing research there has been a connection made that cancer is the result of cells that continually replicate and causes for neoplasm to develop at an alarming rate (2). Neoplasm is the growth of unwanted tissue in some part of the body and is one of the signs of cancer (4). This experiment aims at trying to understand one of the possible mechanisms for this continuous tissue growth.
When observing the factors that play
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The latter has been increasingly studied and it has been found that the disruption of the circadian clock has been related to disorders in humans, such as cancer (2). The focus of this experiment is the role of a specific circadian clock gene on the ability to regenerate cells in planarian. Even prior to birth, from humans down to microscopic bacteria, there are a number of genes that dictate the nearly twenty-four-hour rhythm that develops a process which controls one’s physiology, behavior, and even gene expression, this process is known as the organism’s circadian rhythm (2). Of the many organisms that contain these circadian genes, the one organism that coordinates their rhythm in an interesting manner is the freshwater planarian (Schmidtea mediterranea), which is the model for this experiment. The planarian is a flatworm that eternally maintains its juvenile stage and has a very impressive ability to regenerate, which makes them an excellent model for use in the …show more content…
This is a known procedure for disturbing the function of genes in planarian (4). We made a double stranded timeless RNA and fed it to planarian in order to observe the phenotypic expression. In order to obtain the double stranded DNA a plasmid (pGEM), which contains the DNA sequence for timeless, was a template in a PCR reaction. This process results in multiple copies of the template containing the promoter, T7, at both of the ends. The addition of the promoter allows for identification of the gene and after column purification we were able to perform in vitro transcription via a T7 polymerase, which will then provide the complementary RNA strands, which then lead to the production of dsRNA. In order to insert the dsRNA into the planarian, we will feed the flatworms the dsRNA disguised in liver paste that will then collide with the RNA silencing complex (RISC). This is the process of RNAi and is essentially the key component to knock-down endogenous timeless function. After the dsRNA enters the cells of the planarian it will bind to RISC, which then anti-sense strand will serve as the target for the mRNA. There are then two possible outcomes to what will result of the mRNA: it will either be degraded or its translation will be prevented. Both of the aforementioned situations is due to

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