The Effect Of Imperature On Alkaline Phosphatase Activity

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| |Enzyme Activity |
|Temp |(nmoles pNP.min-1.mL-1) |
|((C) | |
|25 |15.43 |
|30 |16.83 |
|37 |19.61 |
|45 |24.11 |
|60 |14.73 |

Table 3. Effect of substrate concentration on alkaline phosphatase activity
| | |
|Substrate concentration |Enzyme Activity |
|mM pNPP |(nmoles pNP.min-1.mL-1) |
|(complete values in this column) | |
|0.25 |12.17 |
|0.5 |15.76 |
|1.0 |17.62
…show more content…
As the pH of the solution, the enzyme activity of the alkaline phosphatase also increased. From our knowledge of enzymes, and prior research, we understand that individual enzymes each have a corresponding pH at which their activity is at a maximum. This is known as the apparent optimum pH, and varies for each individual enzyme. Our results do not indicate the apparent optimum pH for this reaction, however the enzyme activity of the alkaline phosphatase does increase as the pH increases, which suggests that the apparent optimum pH is greater than or equal to a pH of 11.5.

Effect of temperature on alkaline phosphatase activity
Temperature can have a positive or negative affect on the enzymatic activity of alkaline phosphatase, depending on the extent to which the temperature is increased. In our experiment, as the temperature was increased from 25°C to 45°C, we saw a fairly constant increase in the activity of alkaline phosphatase. This can be seen in our graph ( figure 2). Once the temperature was further increased from 45°C to 60°C however, we saw a decrease in the enzyme activity, such that the enzyme was less active at 60°C than it was found to be at 25°C. From this data we can conclude that the apparent optimum temperature for alkaline phosphatase to be

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