Summary: How Carbon Sources Affect Biofilm Growth

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How Carbon Sources Affect Biofilm Growth
Yeast is single celled and eukaryotic. Biofilms of bacteria can adhere to a device and allow them to withstand environmental changes, which prevents antibiotics from penetrating the cells to kill them. When shifting from a planktonic state to a biofilm, the cells change its proteins. Biofilms produce FLO genes, which code for glycoproteins, cause the yeast to stick together. This is what helps the biofilm form. Once it is forms, the species within the biofilms have cell-to-cell communication, which allows for a division of labor. Biofilms can have both positive and negative effects. Some positive effects include, consuming pollutants in the environment and also brewing various substances such as beer.
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We were given five. Four for treatments and one for the control. divided the plates into three section. The sections were labeled TRY 138, TRY 140, TRY 143. TRY 138 has no modifications to the FLO protein. TRY 140 has a decreased amount of FLO proteins while TRY 143 has an increase in the amount of FLO protein. After the sections were labeled, one member of the group used a sterile toothpick and used that to gently touch the center of the yeast growth of one of the sample strains. The same member then touched the center of the agar surface in the correct section. The membered continued to do this for all plates. Two of the plates contained a fermentable carbon source, maltose. Another two of the plates contained an nonfermentable carbon source, glycerol. The control plate contained glucose in order to show that the fermentable and nonfermentable carbon source do have an effect on how the biofilms growth. The independent variables of this experiment are the carbon source and the amount of FLO gene. The dependent variable is the growth of the yeast. There were many controls in this experiment, one of which is the glucose plate. Furthermore, each agar plate was kept at twenty five degrees Celsius for seven days. In addition, the application and applicator was consistent. In other words, the same member of the group used the same process to apply the yeast to each …show more content…
One of which was from contamination. One section of a plate had multiple fungal growth. This means that the fungus was applied to this section twice. The applicator might have failed to realize that the fungus had already been added and then added more fungus once more. Furthermore, The lack of time is also a source of error. This is because biofilms grow more slowly than planktonic organisms, therefore, more time is needed in order for the biofilms to occur and adhere to the surface. There was not any outliers that effected the overall data. In order to obtain more accurate results, this experiment needs to be set up over a longer time so that the biofilms have a better chance of being produced. In addition to that, being more cautions and alert during the experiment would allow a decrease in human

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