Pros And Cons: Selective Eating And Transgenesis

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It is humans instinct to want to have and create the best of everything. With this drive and scientific knowledge we are able to manipulate a species genetics through several methods. The two methods I will be exploring the process of and the pro’s and con’s is ‘selective breading’ and ‘transgenesis’.

Transgenesis is a form of genetic manipulation where an exogenous gene is introduced into a living organism that is not of the same species as the exogenous gene. This gene will then also be present in the offspring of that organism. An example of this is within corn. The corn is genetically modified to produce its own ‘natural’ pesticide, this is known as ‘BT corn’. The problem with natural corn was that the silk worms liked
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In the step we can manipulate the information to express the Bt gene in a specific way while in the corn. One or more of three gene regions of the Bt gene can be cut apart, replaced or modified. Once we have manipulated and changed to gene, the separate parts fuse back together and form one normal gene however it is now recombinant DNA as there have been changes to the bases sequence. There are four stages that must happen within this stage in order for this to be a successful gene transplant. The promoter is a ‘switch’ which controls when and were the Bt will be expressed in the corn. sequence need to be added for the gene to be placed correctly in the DNA of the corn. For the Bt corn, the promotor used is CaMV35s which comes from the cauliflower mosaic virus. This causes the gene to be active throughout the corns lifespan and to be in most of the plants tissue. The Bt clone gene is also manipulated to enhanced to production of Bt in its cells. For this to happen the corn plants nucleotide G-C will get replaced with A-T from the Bt gene. The termination sequence and a gene marker is the next part involved in modifying the gene. The termination sequence’s job is to inform the cellular machinery that is has reached the end of the gene sequence. What the gene marker does is identify plant cells that carry a specific integration of the transgene. Often it also consist of a protein …show more content…
This stage is where we insert the new modified Bt gene into a single corn plant cell. There are a few methods for this process such as using a gene gun or agrobacterium. The gene gun (micro-projectile bombardment method) is basically where the Bt gene is loaded into a high pressure helium powered gun and fired at the plant cell. This gene will not push through the membrane of the plant cell unless it is connected to very small gold or tungsten particles which will push through the cell membrane and takes the Bt gene in with it. Because corn is a plant, it’s cells are totipotent meaning any single cell form the plant can from a complete new cloned plant. Once the Bt gene is in ta corn plant cell, it becomes part of the corn;s DNA and when the cell divides, all the cells will have the Bt gene.The other method know as agrobacterium tumefaciens is where a bacteria infects the corn cells with a piece of it’s own DNA which results in tumours. This process itself has been genetical modified to switch the tumours into the desired gene for

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