Mitochondrial Dna And Its Effects On The Agar Plate Essay

760 Words Apr 28th, 2016 4 Pages
The result from the transformation process showed no colony growth on the agar plate, which means no plasmid DNA got inserted into the vector (Table 1). Thus, the transformation was considered unsuccessful. With that being said, the gene was not transferred among the bacterial cells. The reason could have been that the agar plate had a strong concentration of antimicrobial drug, so the bacteria may have been unable to grow under such condition. Another reason could be that the cell did not get enough heat shock, or not enough pre-incubation time with CaCl2, which means the bacterial cell wall was not permeable enough for the DNA to come inside and interact with the bacterial DNA.
The DNA gel electrophoresis showed that there were no fragments for both of the PCR samples (Fig 1). However, the restriction digest column showed two fragments promptly at 3000 and 3600 base pairs. By that, it is proved that purified plasmid DNA had the gene of interest, SpHTS, in it. Since the restriction enzyme BsoBI cut the purified plasmid DNA and the SpHTS gene, it should yield two fragments. As expected, the result from the restriction digest process were close to the expected base pairs, which was at 3013 and 3682 basepairs. Therefore, there was SpHTS in the purified DNA and the ligation process was successful. However, the PCR samples were not observed in gel electrophoresis (Fig 1), which means that there were no clone DNA in both PCR samples. However, the concentration of both PCR…

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