Migration Of Cell Experiment

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Experiment 1
In this experiment we definitely observed the migration of cells in all three cells. Cell migration is the movement of cell from one area to another, in response to a chemical signal. The chemical signal is the central to achieving functions; cell differentiation, wound repair, embryotic development and the metastasis of tumors. There is an attraction between the cell and the outer membrane in order for the movement to occur. When participating in the experiment, the cell culture medium was placed in a well the cell serum was added. The cells were incubated at 37 degrees and 5% of CO2, without the CO2 the cells would die. When the incubation occurred, the MitoTracker was also part of it in order for the cells to passively
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The cell samples were treated with three different dyes which were; Phalloidin, DAPI, and Mitoracker. Phalloidin, DAPI, and MitoTracker. Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments. It fluoresces green under the microscope when in the presence of actin (Figure 4.2). DAPI is a blue fluorescent nucleic acid stain, preferentially binds dsDNA and associates with the minor groove AT clusters (Figure 4.3). This dye fluoresces blue when there is the presence of double stranded DNA. The final dye is MitoTracker, which binds to mitochondria and labels them using probes, which fluoresces red under the microscope (Figure …show more content…
Figure 4.2, which was the Phalloidin, was green and definitely showed the structure of actin. The acitin is explained to be very branched which provides the structure for the cell itself. Figure 4.3, which was he MitoTracker since its stain is red when observing under the fluorescence microscope. When observing the image, I could see the rod-shaped rigid structure, which has proven that there are active mitochondria. The last figure is 4.4, which is DAPI, a blue colour is observed indicating the binding to dsDNA. The tiny dots are known to be the nucleus. We know this because the dsDNA is located in the nucleus. There are many world known applications when dealing with this specific experiment. The main application is being able to test diseased and cancerous cells. By testing these cancerous cells allow scientists to research what are/areas these diseases affect. Cell that move quickly and produce more quickly can be observed by using cell migration assay method. For experiment two, the same method can be used to observe if any organelles are present in different species. It is important to see what can organelles respond to especially in the pharmaceutical

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