Cell Migration Lab Report

Experiment 1
In this experiment we definitely observed the migration of cells in all three cells. Cell migration is the movement of cell from one area to another, in response to a chemical signal. The chemical signal is the central to achieving functions; cell differentiation, wound repair, embryotic development and the metastasis of tumors. There is an attraction between the cell and the outer membrane in order for the movement to occur. When participating in the experiment, the cell culture medium was placed in a well the cell serum was added. The cells were incubated at 37 degrees and 5% of CO2, without the CO2 the cells would die. When the incubation occurred, the MitoTracker was also part of it in order for the cells to passively diffuse across the plasma membrane.
When observing, well one contained the most cell migration out of the three wells. Well one contained 30 cells, which was calculated to have 1,800 cells in total, followed by well three which had the total of 1,740 and the second well having the least containing 540 cells in total. We were the first group to prepare our sample therefore allowing more time for the cells to grow. Also, when putting it in the incubator it had went past the designated time, which allowed more
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In well one, 1,800 cells migrated followed by well two which contained 540 cells in total and lastly well three contained 1,740 cells in total. We weren’t surprised by our results because we had a lot of waiting time in order for the cells to grow.
2. All of the dyes were visible under the fluorescent microscope. The green dye stained the actin, the red dye stained for mitochondria and the blue dye stained for dsDNA. If someone would not be able to see these pictures it would indicate contamination and they would not contain organelles and proteins. It can also indicate denaturation during the preparation.
3. This question is answered in the discussion, specifically in the experiment two

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