# Lab Experiment: The Concept Of The Beer-Lambert Law

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In order to complete this lab in a proper manner, the concept of the beer-lambert law must be understood to ensure success in the experiment. the Beer Lambert Law compares the property of light with material through which the light is travelling.4 So, if all the light passes through a solution without any absorption, then absorbance is zero, and the percent transmitted is 100%. If all the light is absorbed, then percent transmittance is zero, and absorption is infinite.3-4 There are many forms of this equation however the formula A=Ɛlc, explains how absorbance is directly proportional to the other parameters, as long as the law is obeyed. A=Ɛlc, also tells us that absorbance depends on the quantity of the compound that is absorbed in the light …show more content…
due to this, one would not be able to get the concentration value of [Cr(NO3)3] because, one would not be able to identify how concentration and absorbance are related and applying this law to the measured absorbance of the equilibrium solution would yield the concentration of the complex. Also without knowing the beer-lambert law, one would not know the outcome of an absorbance vs concentration graph.2 The linear relationship between them is only understood, if one understands the law.

Therefore, the beer-lambert law not only explains the relationship of a solution with light, but it also explains the absorbance and concentration relationship. Without knowing this concept one, would not be able to create the absorbance vs concentration graph as one would not the direct relationship. Due to this, the calculations are needed for this lab would be either inaccurate or
However, above a certain concentration level, the absorbance is no longer proportional to the concentration of the absorbing species, i.e. as the concentration increases, the absorbance does not increase or increases very little. Explain what caused this phenomenon and how can we avoid this error.
The beer lambert law describes the absorption behavior of solution based on low amounts of solutes dissolved in a concentration. However, when the concentration of the absorbing species is too high, the relationship between absorbance and concentration is non-linear. the reason is because5;
1. At high concentrations, the interactions with the solvent and other solute molecules change
2. At high concentrations hydrogen bonding interactions occur which interact differently with the other

• ## Effects Of Intermolecular Forces

This was likely due to the fact that fumaric acid had a large surface area and exhibited intermolecular hydrogen bonding. It had two free –OH groups with which to form hydrogen bonds. Thus, it was greatly attracted to neighboring molecules and required a great amount of energy in order to melt. Maleic acid not have as much surface area with which to interact with the surrounding molecules. Perhaps more importantly, maleic acid also exhibited less intermolecular hydrogen bonding than fumaric acid.…

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• ## Analysis Of Acetone And Toluene

By collecting the distillate at each temperature plateau, fractions containing a single component can be obtained. However, this technique does not work for solutions in which the boiling points of the components differ by less than about 40°C. In such cases, a plot of the temperature versus volume for a simple distillation would rise gradually and would not produce any plateaus. Additionally, the distillate would still contain a mixture of the volatile components, rather than one pure component. In order to separate the volatile components with similar boiling points, multiple simple distillations would have to be performed on the first fraction of distillate obtained, each one producing a more pure sample of the more volatile component.…

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• ## Explain The Empirical Formula Of Magnesium Oxide

(The calculated ratio contains the mistake.) a. Putting in more water than is needed for reaction 3, and then not drying out this excess water. A: Too little oxygen will present in the crucible because oxygen can not dissolve easily in…

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• ## Protein Casein Experiment

[1] [2] 15% acetic acid has lower amount of casein precipitates, indicates that the pH value of casein is faraway to 4.6, too much acids are added and some of the casein precipitates dissolve. It is because more acid adds to the milk; it will decrease the pH of casein more, and less neutral casein will precipitate as more casein will precipitate and dissolve again. [1]…

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• ## Borax And Hcl: Thermodynamic Analysis Of Chemical Equilibria

Solubility increases with high temperature and more agitation (stirring). The less the solution is stirred or if there’s a decrease in temperature, the solution becomes less soluble and forms a solid at the bottom of the solution. The forward reaction is endothermic because the deltaHrxn was positive, meaning that the solution was absorbing heat rather than releasing heat. An increase in Kc shows the increase of solubility in borax solution. If there’s a decrease in Kc values, that means there is a decrease in solubility.…

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• ## Calorimetric Determination Of Enthalpy And Entropy Change

The entropy of the process was determined from the transition enthalpy and temperature values. Based on the positive enthalpy and entropy values, the reaction appears nonspontaneous. The Gibbs free energy, however, is a better indicator or spontaneity. In order to calculate Gibbs free energy, As the temperature increases the Gibbs free energy decreases. This shows a movement towards disorder with increase of temperature.…

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• ## Differences Between Salts And Molecules

Salts and molecules have similar characteristics, and many differences whether we can tell by first looks or not. Testing these two only supports the differences, and many structural functions cause these differences. Since salts are ionically bonded, they have different properties and functions compared to the covalent bonded molecules. Salts have a ionic bond, and we can confirm due to their electronegativity. As you move from left to right on the periodic table you would notice that electronegativity increases.…

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• ## Rehydrating A Hydrate Summary

When the anhydrous sample was rehydrated, only 93.4% of the sample could be recovered. This was because some of the mass of the sample remained stuck to the filter paper and could not be measured in the final mass calculation. This automatically resulted in less mass and did not allow for 100% of the mass to be recovered. 4. If the hydrate would have been overheated and had released a gas, there would have been excess mass released because the mass of the gas that would have been released and water released combined would have been greater than the mass of just the water released.…

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• ## What Is The Conductivity Of C-H Bonds?

If this happened then the solid would never be tested in hexane and would be tested in water instead. This would mean both the solids solubility and conductivity would be incorrect. Secondly, the Bunsen burners does not have a reliable temperature range. This is because a change in oxygen being given to the flame changes how hot the flame is. As a result if the flame was higher or lower than 500℃ the wrong temperature range would be recorded.…

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• ## Reactions Of Alcohol Lab Report

The unknown could be secondary or tertiary based on the Lucas test, but because of the previous results from the Jones test that suggests the unknown cannot be tertiary because then no reaction would have occurred meaning a negative Jones test. Based on both tests the unknown alcohol could only be a secondary alcohol because the tertiary alcohol was eliminated by the result of the Jones testy and the primary alcohol possibility was eliminated by the negative Lucas…

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