Investigation Of A Particle Size Analysis Essay

1118 Words Dec 8th, 2015 5 Pages
RESULTS AND DISCUSSION

Particle size analysis
The diameters of the curcumin, honey and curcumin+WPI+MH complex measured using Mastersizer were 3.480 µm, 1.349 µm and 20.911 µm respectively. The size of WPI measured with Zetasizer was 250 nm. Quantitative particle size and shape analysis provides information that has either a direct effect or correlation to issues such as bioavailability, solubility, formulation stability, and quality control.

Scanning Electron Microscopy
SEM micrographs of curcumin are shown in Fig. 1. SEM analysis showed that curcumin particles were roughly spherical in shape and bound to each other. In a similar way, SEM was done for WPI and honey. But, due to their solubility’s in water; images could not be captured. Pure curcumin particles were reported to be spherical in shape (Modasiya and Patel, 2012). These particles may have versatile advantages for targeted, site-specific delivery purposes as they can penetrate circulating systems and target sites.

Antibacterial activity of curcumin, MH and WPI
Commercial antibiotic (chloramphenicol) used in this study at 30 μg/mL inhibited all the tested pathogens. Curcumin, WPI and MH were tested for their antimicrobial activities against four Gram positive (B. subtilis, L. monocytogenes, S. aureus, and S. pyogenes) and six Gram negative bacteria (E. coli, E. coli O157:H7, P. vulgaris, S. soneii, S. enteritidis, and S. typhimurium). MIC of curcumin against S. aureus, and P. vulgaris were found to be 100…

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