Investigating The Solubility Of Hydrogen Peroxide Essay

1154 Words Oct 23rd, 2015 null Page
Introduction/Hypothesis
Enzymes are catalysts used to speed up reactions and reduce activation energy. These enzymes are important to many bodily functions. Catalase is one of the enzymes essential to cellular health within the body. It is responsible for the breakdown of hydrogen peroxide which, if it builds up, is extremely toxic to cells. One question that scientists have asked and a question that we explore in this lab is what are the optimal conditions for this reaction to occur? This lab was designed to test for the most ideal pH and temperature in which catalase could work most efficiently on a substrate. The enzyme for this lab was a catalase located in cow liver and the substrate was hydrogen peroxide (H2O2). We predicted that catalase would work best when the pH was mas more acidic - specifically at pH 6. The predicted ideal temperature was 27℃. The control for the enzyme test had a solution of neutral pH 7. The control for the temperature test was the solution at room temperature. The independent variables were pH and temperature. The dependent variable was the reaction time of the hydrogen peroxide and the cow liver. A table was created to rank the pHs and the temperatures from fastest reaction time to slowest reaction time. A faster reaction time is an indicator of better and more efficient activity because it means that catalase had an easier time breaking down hydrogen peroxide. This was used to determine which pH the enzyme worked best in. At the end of the…

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