# Goldfish Experiment

1725 Words 7 Pages
Average Metabolic Rate Difference of Goldfish in Dark vs. Ambient Light
Introduction
Metabolism is an interesting field of study to better understand what is happening inside of the body of different organisms that keep them alive. Goldfish (Carassius auratus) were used for this experiment since these small animals metabolism can be easily influenced by different variables. This is due to the fact that goldfish are poikilothermic whose internal temperature is influenced by their surroundings. Oxygen consumption was used to measure the metabolic rate when goldfish are in ambient light as the control and dark as the experimental variable. These goldfish were put into a closed chamber connected to a computer that sensed the oxygen level within
The null hypothesis is that the average metabolic rate of goldfish when examining their oxygen consumption will not be significantly different when goldfish are placed in ambient light versus in the dark.
Materials and Methods
For this experiment, average metabolic rate was measured through oxygen consumption of goldfish when placed in the dark and when in ambient light. First prepare a simplified flowchart of procedure before beginning (Figure 1). To perform this experiment, one needs to obtain two cups of 200 ml of fish water that has sodium bicarbonate, alphatic amine salts and a sodium thiosulfate pentahydrate each and weigh them. Then obtain two goldfish in each cup and then weigh them. After receiving this data, find the weight of the fishes (in grams) which effects metabolism of the fish and so when the database calculates the data, it can take in to account the mass of the fishes. Next get a chamber that is connected to the computer with the LoggerLite program. The chamber has a probe that measures the amount of oxygen in the
ambient light is significant. However, there are only 8 trials for these results so these results are not as accurate as the data that comes from the all trials from all the classes put together, which were 197. From these results the null hypothesis is rejected the experimental hypothesis is supported since the p-value was 0.314, which is much more that 0.05. This proves that the difference in average metabolic rate of goldfish in dark versus ambient light is not significant. The whole class results from all trials gives a better idea about whether metabolism of goldfish is effected when in normal light or in the dark. Based on these results in all trials of the experiment, the metabolic rate of the goldfish was higher in ambient light that in the dark since average change in oxygen concentration was bigger that of the average change in oxygen consumption in the dark. The difference in average change in oxygen consumption in ambient light versus in the dark was about 16 mgO2 /(L*hr*kg). These results appear to support the findings in the experiment by Borniger, where they found that in a little bit of light, there was decrease in metabolic rate of mice (Borniger et al., 2014). Also 16 mgO2 /(L*hr*kg) is not a big difference of change in mean metabolic rate by the

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• ## Zebra Fish Essay

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• ## Goldfish Metabolism

In a separate beaker of 200 ml of fish water, a 50mg caffeine tablet was dissolved. To accurately test the effect of caffeine on oxygen consumption, the same two fish were used for both the control and experimental trials (Trial 1). After receiving our data from LoggerLite and transferring it on an Excel sheet, 100mL of fish water was removed from the oxygen chamber and 100 mL of caffeine-treated fish water was added to the chamber. Once the plunger was placed back on the oxygen chamber, the fish were left to acclimate to their new environment for 3 minutes. As soon as the fish were acclimated, LoggerLite began taking new measurements of oxygen consumption in 20 second intervals.…

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