Effect Of Metabolism On Goldfish Metabolism

Superior Essays
Caffeine Impact on Goldfish Metabolism
Introduction
Metabolism is a process that enable organisms to maintain their life. Metabolism can be measured in numerous parameters. Within this experiment in order to measure the metabolism rate by determined the level of dissolved oxygen. Goldfish is an example of poikilotherm that are organisms that are influenced by the environment surrounding them. Thus, the manipulation of surrounding can influence the metabolism rate due them being ectotherms. However, fish can build up tolerances and can acclimate to different environments with varying oxygen levels (Prosser, C. L. et al, 1957). Through acclimation, the fish have developed tolerance to hypoxia and by its oxygen consumption as a function
…show more content…
The warm up of the probe is done through the LoggerLite program under the file metabolism experiment. The chamber needs to be filled with 200 mL fish water while the probe is calibrating. During the probe calibration, weight a separate container with 200 mL fish water on the analytical balance. Then obtain two similar sized goldfish and weight the goldfish with the analytical balance already tare with the weight of the container and 200 mL fish water. Record down the weight of the 2 goldfish. Transfer everything from the container into the chamber. Close the chamber by pushing the cap until there is some water leaking out on top in order to get rid of the bubbles. Let the goldfish acclimate to their environment for at least 3 minutes before measurements are …show more content…
Click on the Collect button to collect measurements of the chamber. Let the recording of measurement for 30 to 60 seconds before clicking the Stop button stop the test run. Clear the test run before conducting the trial one. Repeat the whole process again for the control run, but for 10 minutes. During the run, note down the goldfish behavioral activity. For the experimental rule, replenish the deoxygenated fish water with caffeine fish water to investigate the influence that caffeine can have on the fish metabolism. Take out around 100 mL of deoxygenated fish water from the chamber. Dissolve 50mg of caffeine tablet within 100 mL of fish water. Make sure that the caffeine tablet is dissolved finely by stirring the solution with a rod. Add in the caffeine-treated fish water into the chamber replacing the fish water that was taken out. Follow the procedures described for the control run to take down the D.O. measurements. For the second trial, is the repeat of the first trial the only difference is the subjects. The goldfish need to be switch out with new goldfish that haven been expose to caffeine in order to eliminate the factor of chronic exposure of caffeine could potentially have on the metabolism rate. Since this experiment focuses on acute caffeine

Related Documents

  • Improved Essays

    Gel Solution Lab Report

    • 844 Words
    • 4 Pages

    To make the desired buffer, another 100 ml beaker was used to prepare a solution buffer of 50 ml. Following the prelab calculations once again and using the weighting balance, the amount of Tris and SDS needed was weighted. The Tris used were 3.206g and SDS 0.205g. The solids were added to the beaker and to this 50 ml of water. The beaker was stir on the stirring plate until the solution dissolved completely.…

    • 844 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    HCl slowly with stirring until the mixture is acidic to congo red paper. Collect the resulting crystalline precipitate of hippuric acid or benzoyl glycine , which contaminated with a little benzoic acid upon a Buchner funnel, wash with cold water and dry. Place the solid in the 100 ml beaker with 10 ml of CCl4 ,cover the beaker with the watch glass and boil the mixture gently for 5 minutes. Allow the mixture to cool slightly and filter by gentle suction and wash it 3-4 times with CCl4. Recrystallize it from the boiling water.…

    • 2234 Words
    • 9 Pages
    Decent Essays
  • Improved Essays

    Bacillus Subtilis

    • 1799 Words
    • 8 Pages

    This is known as a pour plate. Use a pipette and filler (or syringe) to place 9cm3 distilled water into empty bottles B and C. Using the same pipette, transfer 1cm3 antiseptic from bottle A to bottle B. Gently shake B to mix the contents, then transfer 1cm3 from B into…

    • 1799 Words
    • 8 Pages
    Improved Essays
  • Superior Essays

    Elisa Research Paper

    • 1444 Words
    • 6 Pages

    Make sure that the centrifuge tubes are properly balanced. If the centrifuge tubes do not have pellets at the bottom centrifuge again. Once back in the biosafety cabinet, remove the supernatant at the top of the centrifuge tubes until only the pellet remains. Discard the supernatant into the organic waste flask. Then in a well add trypan blue.…

    • 1444 Words
    • 6 Pages
    Superior Essays
  • Decent Essays

    Bacteria Lab Report

    • 343 Words
    • 2 Pages

    The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder. Dissolve completely the nutrient broth powder in beaker containing 200 ml of distilled or the deionise water.…

    • 343 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Goldfish Metabolism

    • 1824 Words
    • 8 Pages

    In a separate beaker of 200 ml of fish water, a 50mg caffeine tablet was dissolved. To accurately test the effect of caffeine on oxygen consumption, the same two fish were used for both the control and experimental trials (Trial 1). After receiving our data from LoggerLite and transferring it on an Excel sheet, 100mL of fish water was removed from the oxygen chamber and 100 mL of caffeine-treated fish water was added to the chamber. Once the plunger was placed back on the oxygen chamber, the fish were left to acclimate to their new environment for 3 minutes. As soon as the fish were acclimated, LoggerLite began taking new measurements of oxygen consumption in 20 second intervals.…

    • 1824 Words
    • 8 Pages
    Improved Essays
  • Superior Essays

    Stir the KNO3 in the distilled H2O until all the KNO3¬ dissolves. 5. Remove the test tube from the water bath and keep stirring the contents until potassium nitrate crystals start to appear. 6. Record the temperature this occurs at and continue stirring until all of it has crystallized again.…

    • 1221 Words
    • 5 Pages
    Superior Essays
  • Great Essays

    After the gel has been allowed to run for about an hour, the isoelectric tube will be rinsed with running buffer and set aside. Next, an SDS gel will be prepared in order to separate the proteins based on mass. This will be done by sandwiching two spacers between two glass plates and placing them within a plastic bag. The plate/bag assembly will then be placed into a casting stand and filled with a solution consisting of 12% gel mix, 80 microliters of 100 mg/ml ammonium persulfate, and 15 microliters of TEMED. Next, a comb will be wedged between the two plates and the solution will be allowed to solidify.…

    • 1330 Words
    • 6 Pages
    Great Essays
  • Superior Essays

    Double Salt Lab Report

    • 1556 Words
    • 7 Pages

    They added about 20 mL of deionized water to the salt and stirred the solution until it dissolved. As it dissolved, the solution turned into a light blue color. Then to make sure that very little of the solution was left on the stirring rod, they rinsed it with more deionized water into the beaker. After thoroughly rinsing a 50-mL volumetric flask with deionized water, they transferred the solution into this flask and rinsed the 100-mL beaker with deionized water in order to ensure as much of the solution was transferred to the flask as possible. They filled the remainder of the flask with more deionized water, placed a rubber stopper into the top of the flask, and inverted it 20 times in order to mix the solution.…

    • 1556 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Goldfish Experiment

    • 1725 Words
    • 7 Pages

    Then obtain two goldfish in each cup and then weigh them. After receiving this data, find the weight of the fishes (in grams) which effects metabolism of the fish and so when the database calculates the data, it can take in to account the mass of the fishes. Next get a chamber that is connected to the computer with the LoggerLite program. The chamber has a probe that measures the amount of oxygen in the…

    • 1725 Words
    • 7 Pages
    Superior Essays