Behavioral measurements.
Force swimming test (FST). Each mouse was placed into a 20cm diameter glass cylindrical beaker that was filled with warm water (25-30°C) and allowed to swim freely for 6 min. All the experiments were recorded by video. We analyzed the immobility time in last 4 min.
Tail suspension test (TST). Each subject was suspended at a hook with tape for 6 min and recorded by video. The immobility time was analyzed in last 4 min.
Elevated plus maze (EMP). In the beginning of the experiment, each mouse was placed at the junction of open arms and closed arms facing to same open arms. The activities of mice were recorded for 10min by video. The time spent in open arms, closed arm and central time was analyzed.
Object location test
Force swimming test (FST). Each mouse was placed into a 20cm diameter glass cylindrical beaker that was filled with warm water (25-30°C) and allowed to swim freely for 6 min. All the experiments were recorded by video. We analyzed the immobility time in last 4 min.
Tail suspension test (TST). Each subject was suspended at a hook with tape for 6 min and recorded by video. The immobility time was analyzed in last 4 min.
Elevated plus maze (EMP). In the beginning of the experiment, each mouse was placed at the junction of open arms and closed arms facing to same open arms. The activities of mice were recorded for 10min by video. The time spent in open arms, closed arm and central time was analyzed.
Object location test