Discussion:
This experiment was designed to understand how manipulating protein expression would change C. elegans’ level of chemo sensation. Based on the data generated from our individual data and the class data, the data did not match the theoretical numbers. Only our individual syd-1 data was able to exactly match with the theoretical chemotaxis index.
There are many potential reasons why the worms did not move towards the diacetyl, however, it was surprising how syd-1expressed such a strong experimental chemotaxis of 1.00 compared to even our negative control which had a value of 0.583. Our second experimental variable dpy-2 had an experimental chemotaxis of 0.737. These two chemotaxis values had a higher chemotaxis value than our negative control, which originally was supposed to be as close to 1.00 as possible. Observing the chemotaxis of syd-1 and contrasting it to the negative control, one may infer that reducing syd-1 might increase chemo sensation towards the diacetyl. This data is only proven through our own individual data and does not account for the data for the whole class. However, the data produced by the whole class had a higher …show more content…
With this data, I predicted that reducing such a protein should not affect the chemotaxis values because if the C. elegans were to move towards the diacetyl anyway, then the lack of motor neurons would not cause the chemotaxis levels to decrease nor increase. The worms would still move towards the diacetyl. Comparing our individual data with the average chemotaxis index of the University, our values aligned with the theoretical values. There is no certain conclusion for the syd-1 because the levels of chemotaxis index increased in contrast to the negative control as well. Our individual data showed that the C. elegans actively moved towards the
This experiment was designed to understand how manipulating protein expression would change C. elegans’ level of chemo sensation. Based on the data generated from our individual data and the class data, the data did not match the theoretical numbers. Only our individual syd-1 data was able to exactly match with the theoretical chemotaxis index.
There are many potential reasons why the worms did not move towards the diacetyl, however, it was surprising how syd-1expressed such a strong experimental chemotaxis of 1.00 compared to even our negative control which had a value of 0.583. Our second experimental variable dpy-2 had an experimental chemotaxis of 0.737. These two chemotaxis values had a higher chemotaxis value than our negative control, which originally was supposed to be as close to 1.00 as possible. Observing the chemotaxis of syd-1 and contrasting it to the negative control, one may infer that reducing syd-1 might increase chemo sensation towards the diacetyl. This data is only proven through our own individual data and does not account for the data for the whole class. However, the data produced by the whole class had a higher …show more content…
With this data, I predicted that reducing such a protein should not affect the chemotaxis values because if the C. elegans were to move towards the diacetyl anyway, then the lack of motor neurons would not cause the chemotaxis levels to decrease nor increase. The worms would still move towards the diacetyl. Comparing our individual data with the average chemotaxis index of the University, our values aligned with the theoretical values. There is no certain conclusion for the syd-1 because the levels of chemotaxis index increased in contrast to the negative control as well. Our individual data showed that the C. elegans actively moved towards the