6. IN-VIVO STUDY
Inflammation is a protective attempt by the organism to remove the injurious stimuli and to initiate the healing process. Inflammatory diseases are very common throughout the world. Rheumatoid arthritis is one of the oldest known diseases of mankind affecting the majority of population no substantial progress has been made in achieving a permanent cure and different types of rheumatic diseases are a major cause of morbidity of the working force.
Inflammation results in the liberation of endogenous mediators like histamine, serotonin, bradykinin, prostaglandins etc. These mediators even in small quantities can elicit pain response. Anti inflammatory drugs make up about half of analgesics, relieving pain by reducing inflammation.
Inflammation is a physiological reaction to injury or to infectious, allergic, or chemical irritation. Inflammatory processes are complex biochemical phenomena characterized by tissue edema, pain, and leukocyte infiltration (Staff ,Bielory …show more content…
The rats were anesthetized with light ether anaesthesia. Under aseptic conditions two sterilized cotton pellet (10mg) were implanted subcutaneously on either side of lumbar region in each rat. The incisions were sutured by silk 2.0 sutures and the wounds were sealed with betadine solution to prevent contamination. Bleeding was minimal and the animals recovered within 5 - 10 min from the effect of anaesthesia. All the groups were treated with drugs daily for 7 days including the day of implantation of pellets. On the eighth day the animals were anaesthetized with ether, the cotton pellets were removed and dried at 60 0C for 24 h. The dry weight of the granuloma was calculated by noting the difference in the dry weight of the cotton pellets recorded before and after implantation. The incisions were sutured by silk 2.0 sutures and sealed with betadine solution and animals were
Inflammation is a protective attempt by the organism to remove the injurious stimuli and to initiate the healing process. Inflammatory diseases are very common throughout the world. Rheumatoid arthritis is one of the oldest known diseases of mankind affecting the majority of population no substantial progress has been made in achieving a permanent cure and different types of rheumatic diseases are a major cause of morbidity of the working force.
Inflammation results in the liberation of endogenous mediators like histamine, serotonin, bradykinin, prostaglandins etc. These mediators even in small quantities can elicit pain response. Anti inflammatory drugs make up about half of analgesics, relieving pain by reducing inflammation.
Inflammation is a physiological reaction to injury or to infectious, allergic, or chemical irritation. Inflammatory processes are complex biochemical phenomena characterized by tissue edema, pain, and leukocyte infiltration (Staff ,Bielory …show more content…
The rats were anesthetized with light ether anaesthesia. Under aseptic conditions two sterilized cotton pellet (10mg) were implanted subcutaneously on either side of lumbar region in each rat. The incisions were sutured by silk 2.0 sutures and the wounds were sealed with betadine solution to prevent contamination. Bleeding was minimal and the animals recovered within 5 - 10 min from the effect of anaesthesia. All the groups were treated with drugs daily for 7 days including the day of implantation of pellets. On the eighth day the animals were anaesthetized with ether, the cotton pellets were removed and dried at 60 0C for 24 h. The dry weight of the granuloma was calculated by noting the difference in the dry weight of the cotton pellets recorded before and after implantation. The incisions were sutured by silk 2.0 sutures and sealed with betadine solution and animals were