Blood Physiology Lab Report

1201 Words 5 Pages
Sarah Hill
BIOL 3830H F03
Simon Tapper
Wednesday, October 11th, 2017

The relationship between blood physiology and cardiac ability in Equus caballus compared to Bos taurus and Capra aegagrus hircus compared to Canis lupus familiaris

Abstract The purpose of this experiment was to study the relationship between blood physiology and the ability to transport oxygen. The red blood cell content, hematocrit percent, and hemoglobin concentrations of Equus caballus (horse) and Bos taurus (bovine) were compared as well as Capra aegagrus hircus (goat) and Canis lupus familiaris (dog). It was hypothesized that animals with higher mean cell volume (MCV), mean cell hemoglobin concentration (MCHC), and mean cell hemoglobin (MCH) would have a higher aerobic capacity because they would have larger red blood cells and
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The hematocrit was read using the provided scale and the plasma colour was noted. The hematocrit was calculated as (height of red blood cell column)/(height of entire column) x100.4 To determine the hemoglobin concentration, a drop of blood from each of the species, horse, bovine, goat, and dog, was added to a piece of Tallquist blotting paper, and the colour of blood was compared to the "Tallquist colour standard" to determine the hemoglobin percent.4 To summarize the findings, mean cell volume (hematocritnumber of red blood cells), mean cell hemoglobin concentration (Hemoglobinhematocrit), and mean cell hemoglobin (Hemoglobinnumber of red blood cells) were calculated. ANOVA software was used to determine the P value which represents if there is a significant difference between the data sets being compared. "Post-hoc analysis using Tukey's test" is used when a significant difference is found using the ANOVA software to determine which groups are being compared by ANOVA data.4

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