Enterobacter Aerogenes And Disadvantages Of Microorganisms

1913 Words 8 Pages
I. INTRODUTION
The worldwide the bacterial community includes billions of microorganisms. Microbiologist and other scientists are constantly working of identifying species and classifying them by morphological, biochemical and molecular tests. This is valuable to the medical community, as some of these microorganisms are pathogens, but also to other industries such as pharmaceuticals, foods and drinks production, ecological, agriculture and biotechnology as humans have used bacteria for years to fulfill their needs.
Likewise, morphological and biochemical testing were performed to identify the bacterial unknown for this project out of three possible options: Enterococcus faecalis, Enterobacter aerogenes, and Serratia marcescens. After careful
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A positive result is indicated by the production of blue pigmentation along the streak line. Based on the results, the test would confirm E. aerogenes and eliminate S. marcescens and E.faecalis. A red pigmentation represents growth of S.marcescens. However the pigment produced was a burgundy, dark almost purple red, which indicated that a change in color was occurring. Lipase production is highly dependent on optimizing pH, correct temperature and incubation time. It is possible that the sample was not incubated at 25° C, which would not be the optimal temperature or the pH could have been imbalanced causing a slower …show more content…
aerogenes, E. faecalis and S.marcescens. After running multiple physiological, and biochemical tests with the intention of eliminating the other two possibilities, with the exception of the fat hydrolysis and motility test (both has been explained in detail in the discussion section) all other result confirmed sample #14 as S. marcescens. Furthermore, the molecular results also confirm the tests ran in the lab and reinforce the results. It is very important to be able to run multiple tests to confirm the results obtained, as bacterial mutations, and multiple human errors can occur. Additionally, having the opportunity to run the 16S rRNA coding region strengthen the case that supports the hypothesis that the unknowns is the opportunistic pathogen S.

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