Developing Two Bioanalytical Sensors As A Means For Study Cellular Phenomena

1652 Words Nov 29th, 2016 7 Pages
The aim of our project is to develop two bioanalytical sensors as a means to study cellular phenomena in organized cellular 3D microenvironments that closely mimic biological habitats. Three important questions, listed below, will be addressed during this project, which we discuss in detail in Sections 3.1-3.2.

(1) Can we determine concentrations of cellular biogenic species (NO, H2O2) in organized microcommunities using T-CUAs?
(2) Can we monitor proximity-dependent cellular behavior in dynamic microcolonies?
(3) Can we perform real-time analyses of cellular behavior in response to in vivo environments?

3.1 T-CUAs for the Electrochemical Detection of Cellularly Derived Biogenic Species in Organized 3D Microenvironments.

The multifaceted environments in which cells interact can be highly differentiated and susceptible to changes that influence cellular survival, division, differentiation, and death. Such dynamic responses are often mediated, at least in part, by changes in the concentration of signaling biogenic molecules. Hence, the detection of these chemical species is key to understanding cellular communication. Using T-CUAs as electrochemical sensors in tandem with ยต3DP cell culture, we will electrochemical detect two signaling molecules, NO and H2O2, directly derived from macrophages and S. gordonii cells, respectively.
Macrophages are immunological, eukaryotic-type cells that respond to bacterial infections within in an organism by secreting high concentration of…

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