Design Two Bioanalytical Sensors As A Means For Study Cellular Communication And Behavior
The aim of our project is to design two bioanalytical sensors as a means to study cellular communication and behavior in organized cellular 3D microenvironments that closely mimic in vivo settings. Three important questions, listed below, will be addressed during this project, which we discuss in detail in Sections 3.1-3.3.
(1) What concentrations of signaling molecules (NO, H2O2) do cells in organized in 3D microenvironments secrete?
(2) How does bacterial behavior in bacterial 3D microenvironments change in different chemical environments?
(3) How does bacterial behavior in model cellular 3D microenvironments change in in vivo surroundings?
3.1 T-CUAs for Electrochemical Detection of Cellularly Derived Biogenic Molecules in Organized 3D Microenvironments.
The multifaceted environments in which cells interact can be highly differentiated and susceptible to changes that influence cellular survival, division, differentiation, and death. Such dynamic responses are often mediated, at least in part, by changes in the concentration of signaling biogenic molecules. Hence, the detection of these chemical species is key to understanding cellular communication. Consequently, we will detect cellularly derived signaling molecules using T-CUAs as electrochemical detectors. We plan to integrate µ3DP to micropattern on the surface on the T-CUAs. This will allow us to monitor changes in electrochemical signal from cells in organized 3D microenvironments, which…