Summary Of Botulinum Toxin As A Biological Weapon

Decent Essays
Arnon, S. S., R. Schechter, T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eit-zen, A. D. Fine, J. Hauer, M. Layton, S. Lillibridge, M. T. Osterholm, T. O. Toole, G. Parker, T. M. Perl, P. K. Russell, D. L. Swerdlow and K. Tonat (2001). Botulinum toxin as a biological weapon: medical and public health management. The Journal of the American Medical Association285: 1059-1070.
Bhambhani, A. and C. V. Kumar (2006). Protein/DNA/inorganic materials: DNA binding to layered α-Zirconium phosphate enhances bound protein structure and activity. Advanced Materials18: 939-942.
Bhambhani, A. and C. V. Kumar (2008). Enzyme-inorganic nanoporous materials: differential scanning calorimetric studies and protein stability. Microporous and
…show more content…
V. and A. Chaudhari (2000). Proteins immobilized at the galleries of layered α-Zirconium phosphate: structure and activity studies. Journal of American chemical society122: 830-837.
Kumar, C. V. and A. Chaudhari (2002). High temperature peroxidase activities of HRP and hemoglobin in the galleries of layered Zr(IV)phosphate. Chemical Communications 2382-2383.
Kumar, C. V. and G. L. McLendon (1997). Nanoencapsulation of Cytochrome c and horseradish peroxidase at the galleries of α-Zirconium phosphate. Chemistry of Materials9: 863-870.
Kwon, S. J., E. J. Jeong, Y. C. Yoo, C. Cai, G.- H. Yang, J. C. Lee, J. S. Dordick, R. J. Linhardt, and K. B. Lee (2014). High sensitivity detection of active Botulinum Neurotoxin by glyco-quantitative polymerase chain-reaction. Analytical Chemistry86: 2279-2284.
Ladd, J., A. D. Taylor, J. Homola and S. Jing (2008). Detection of Botulinum Neurotoxins in buffer and honey using a surface plasmon resonance (SPR) sensor. Sensors and Actuators B: Chemical130: 129-134.
Liu, H., C. Queffelec, C. Charlier, A. Defontaine, A. Fateh, C. Tellier, D. R. Talham and B. Bujoli (2014). Design and optimization of a phosphopeptide anchor for specific immobilization of a capture protein on zirconium phosphonate modified supports. Langmuir30:

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