Parasitoid Rearing Case Study

Amazing Essays
Progress summary
With the termination of sterile insect technique efforts to limit the spread of Argentine Cactus Moth, Cactoblastis cactorum, population management strategies, such as biological control, are being investigated. The parasitoid Apanteles opuntiarum, is believed to have co-evolved with C. cactorum. To assess the non-target effects of the koinobiont larval parasitoid on non-target pyralids, host specificity tests were implemented in quarantine laboratory conditions.
Argentine Cactus Moth (C. cactorum) Rearing
The laboratory filter colony maintained at the Biological Control Rearing Facility in Gainesville, Florida produced 680,679 cactus moth pupae during FY 14-15. Fifty-three lots (lot = ≈300 cups) were infested, and the average number of eggs per cup was sixty. Pupae were collected after an average of forty-one days had passed. Forty-seven of these lots had completed pupal development during this time interval and the lot pupation percentage ranged from 42.1-92.4 with the majority of the lots being over 77% pupal development.
Disease management continued to be a critical
…show more content…
Collaborators sent a number of native North American cactus moth larvae from different locations in Texas, Arizona, and New Mexico to the containment facility in Gainesville for non-target assays. So far no parasitization occurred in no-choice, non-target host testing of the other Lepidopteran species. Individuals from colonies of Melitara prodenialis from ARS lab, Tallahassee were used in non-target testing. Out of 501 M. prodenialis larvae, none of them parasitized; 123 successfully completed pupation and 378 died. Preliminary research has indicated that A. opuntiarum appears to be host specific to C. cactorum. We will finalize host range testing to confirm that it has the same degree of host specificity as the Argentine populations have previously studied in the

Related Documents

  • Decent Essays

    Salamanders Case Study

    • 745 Words
    • 3 Pages

    There were 20 male salamanders that were chosen at random to participate in experiment one. Although the males were chosen at random; however, the females were not. There were 60 females collected for this experiment were not used in any courtship trials before the start of the experiment. The female salamanders were placed into three distinct groups: nongravid, weakly gravid, or strongly gravid salamanders. Female salamanders measuring less than 46 mm were not used in the experiment.…

    • 745 Words
    • 3 Pages
    Decent Essays
  • Decent Essays

    Analysis Of HCV DNA

    • 975 Words
    • 4 Pages

    Approach: The specific aim of this Phase I project is to establish the technical merit and feasibility of detection of HCV RNA using an innovative assay called TARA. The overall work principles and design of the assays are shown in Figure 1. We will use peptide nucleic acid (PNA)-based probes to investigate in detail the signaling power and the selectivity of a transfer reaction (Figure 2). PNA is a chemically and biologically stable DNA analogue that combines an increased affinity towards DNA (RNA) with a hybridization that is highly sequence selective [28]. Due to modifications in its backbone, it does not degrade in the presence of proteases such as Proteinase K. Sequence-specific PNA oligonucleotides are labeled with antigen (biotin, or…

    • 975 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Pseudomonas Syringae

    • 1648 Words
    • 7 Pages

    It has been suggested that chromosome 14 carries the primary determinants for adaptation of F. oxysporum towards tomato. The effector genes are conserved in…

    • 1648 Words
    • 7 Pages
    Decent Essays
  • Decent Essays

    Chlordane Case Study

    • 1172 Words
    • 5 Pages

    The fatal human dose is expected to be 6g and convulsive symptoms occur with an oral amount of 2.25g. In a human exposure case study by the Department of National Health and Welfare, there were no illness found among workers that were exposed to chlordane from one to three years. Examination of 22 workers showed no signs of symptoms that were found in animals that were exposed in labs. Some observable symptoms that should have been found in the workers that were seen in the exposed rats included but not limited to loss of weight, anorexia, nervous disorder, and disturbance of vision and respiration. However, none of these signs were seen in the workers over a long period of constant exposure.…

    • 1172 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    Desert Iguana Summary

    • 1182 Words
    • 5 Pages

    One weakness in particular was that this article presented a small sample size for the iguanas. The researches began with 33 dessert iguanas, but only ended up using 16 for the experiment, 9 males and 7 females. They eliminated a significant number of iguanas from the study by running prior tests and examinations. Also, the researches did not address data regarding sex based behavioral responses, I would personally find it fascinating to know whether the different sexes had relatively different behavioral frequencies when exposed to the different…

    • 1182 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    Duncan possibly came in contact with over 100 people. His fiancé, her son, her 2 nephews, the ambulance crew, a handful of school children and 48 other people were put into different quarantines for 21 days. Symptoms of Ebola takes 8-10 days to appear after exposure. The incubation period for Ebola is 2-21 days. Ebola can also be found in semen up to 3 months after treatment.…

    • 816 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Bluetongue Case Study

    • 1832 Words
    • 8 Pages

    In Egypt, there is no vaccination program is running or used, so positive serum samples means that BTV-specific antibodies which are still circulating in the tested animals without any detectable signs is due to subclinical infection (Maclachlan et al., 2009). Although in 2008 Egypt declared free from BT, it has notice the presence of BT infection in the last few years. No available data on disease history in Egypt. The disease has become a threatening disease as it may attack animals in the form of massive outbreaks due to animal exportation during Eid Al Adha, which is a major feast in Egypt and due to the climate change as high temperature and humidity (Kawther, 2012).This study estimates the prevalence and distribution of antibodies to BTV in different localities at Assiut governorate, Egypt. Our results revealed high seroprevalence…

    • 1832 Words
    • 8 Pages
    Decent Essays
  • Decent Essays

    Arthropods, like mosquitoes, mites and lice, are the main vectors for EEE, WEE and VEE. These arthropods feed on the bird and rodent populations, which are the carriers, and transmit it to the victim animal, which are mainly horses. Interestingly enough, while not a vector for transmission, reptiles, such as snakes, have been known to be carriers over winter months. It is important to note that the arthropod vector is essential for spread of this disease. There are no known incidents of VEE or WEE being spreadable from a horse to another horse or human, and EEE is a dead end as well in this regard, except for in laboratory demonstrations.…

    • 1335 Words
    • 6 Pages
    Decent Essays
  • Decent Essays

    Pest Of Brinjal Essay

    • 1038 Words
    • 5 Pages

    Corn earworm causes damage to the leaves, flower, buds, pods and beans. Control methods • Application of Bacillus thuringiensis to control insects organically on grown plants. Farmers should monitor plants for eggs and larvae and also natural enemies that could affect the crops. 3. Mexican bean bettle • Mexican beetle are orange-brown in colour with black spots.…

    • 1038 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    GMO Argument Essay

    • 570 Words
    • 3 Pages

    GM crop, becoming resistant to the pesticides. Based on Brandon Keim’s article, farmers had found rootworms that are resistant to pesticide. Rootworm feed on roots of plants, causing damage to crops, results loss for farmers. Furthermore, GMO harms the environment by contaminating nearby aquatic life. Genetically modified crops’ toxic characteristics can kill off aquatic insects.…

    • 570 Words
    • 3 Pages
    Decent Essays