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Introduction to the Chemical Analysis of Water Samples

Each group will collect a fresh water sample from campus according to directions provided form your instructor or TA.

You will learn how to use several pieces of equipment and several chemical kits in order to analyze the water sample. Each group may split up the tasks however it sees fit- however, each person in the group is expected to be able to complete any and all of the tests performed, with directions of course! Make sure that you answer the question on the last page of this handout as you work through the lab. You will need to share your answers with the others in your group.

Test to Perform | Equipment or Kit Used | pH | Smart Colorimeter | Turbidity | Smart
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Dissolved Oxygen (ppm)

Directions for Conductivity:

1. You will use the Vernier LabQuest 2, and conductivity probe for this test. It will give you an indication of the number of ions in the water sample (conductivity is also proportional to salinity) Make sure the LabQuest2 is plugged in and set up properly. 2. Make sure the switch on the conductivity probe is connected to the LabQuest and set to probe to the 0 – 2,000 μS range. 3. Place sample water in a beaker. 4. Place conductivity probe into your water sample. 5. Tap the green triangle at the bottom left of the LabQuest to collect the data for conductivity.

Directions for Fecal Coliform Test:

1. Obtain one coated Petri dish and one vial of coliscan easygel liquid media from your TA. Keep the Petri dish closed! 2. Using a sharpie write your lab day and time, your group number, the date and the sample location (Library Pond) on the TOP of the Petri dish around the edge (see the diagram on the blackboard). 3. Using a pipet and pipet pump (your TA will demonstrate how to use these) place 1 ml of your water sample into the vial of media, and GENTLY swirl to mix (there is no need to put the cap back on the vial of media). 4. Then, using one hand, carefully crack open the lid of the Petri dish (do NOT remove completely) and then pour in the contents of the vial.

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